Aspirin and indomethacin reduce lung inflammation of mice exposed to cigarette smoke

被引:19
|
作者
Castro, Paulo [2 ]
Nasser, Helena [2 ]
Abrahao, Agessandro [2 ]
dos Reis, Larissa Cardilo [2 ]
Rica, Ingred [2 ]
Valenca, Samuel S. [3 ]
Rezende, Daniele C. [1 ]
Quintas, Luis E. M. [1 ]
Cavalcante, Moises C. Marinho [2 ]
Porto, Luis Cristovao [3 ]
Koatz, Vera Lucia G. [2 ]
机构
[1] Univ Fed Rio de Janeiro, ICB, Lab Farmacol Bioquim & Mol, CCS, BR-21941902 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Bioquim Med, BR-21941 Rio De Janeiro, Brazil
[3] Univ Estado Rio De Janeiro, Inst Biol, Dept Histol & Embriol, Rio De Janeiro, Brazil
关键词
Cigarette smoke; Aspirin; Indomethacin; Lung inflammation; NF-kappa B; p38 MAP kinase; NF-KAPPA-B; NONSTEROIDAL ANTIINFLAMMATORY DRUGS; PROLIFERATOR-ACTIVATED RECEPTOR; CARRAGEENAN-INDUCED PLEURISY; P38 MAP KINASE; CYCLOOXYGENASE INHIBITORS; PULMONARY INFLAMMATION; ALVEOLAR MACROPHAGES; INDUCED EMPHYSEMA; NITRIC-OXIDE;
D O I
10.1016/j.bcp.2008.12.012
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Neutrophil accumulation response to cigarette smoke (CS) in humans and animal models is believed to play an important role in pathogenesis of many tobacco-related lung diseases. Here we evaluated the lung anti-inflammatory effect of aspirin and indomethacin in mice exposed to CS. C57BL/6 mice were exposed to four cigarettes per day during 4 days and were treated i.p. with aspirin or indomethacin, administered each day 1 h before CS exposure. Twenty four hours after the last exposure, cells and inflammatory mediators were assessed in bronchoalveolar lavage (BAL) fluid and the lungs used for evaluation of lipid peroxidation, p38 mitogen-activated protein kinase (MAPK) phosphorylation and nuclear transcription factor kappa B (NF-kappa B) activation. Exposure to CS resulted in a marked lung neutrophilia. Moreover, the levels of oxidative stress-related lipid peroxidation, prostaglandin E-2 (PGE(2)), interleukin 1 beta (IL-1 beta), monocyte chemotactic protein 1 (MCP-1), and activated NF-kappa B and p38 MAPK were greatly increased in CS group. Aspirin or indomethacin treatment led to a significant reduction of neutrophil influx, but only aspirin resulted in dramatic decrease of inflammatory mediators. Moreover, both drugs reduced lung p38 MAPK and NF-kappa B activation induced by CS. These results demonstrate that short-term CS exposure has profound airway inflammatory effects counteracted by the anti-inflammatory agents aspirin and indomethacin, probably through COX-dependent and -independent mechanisms. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:1029 / 1039
页数:11
相关论文
共 50 条
  • [1] PROPOLIS REDUCED ACUTE LUNG INFLAMMATION IN MICE EXPOSED TO CIGARETTE SMOKE
    Lopes, A. A.
    Ferreira, T. S.
    Nesi, R. T.
    Alves, J. N.
    Bandeira, A. C. B.
    Melo, A. C.
    Trajano, E. L.
    Porto, L. C.
    Valenca, S. S.
    WOUND REPAIR AND REGENERATION, 2009, 17 (04) : A73 - A73
  • [2] Apocynin and ebselen reduce influenza A virus-induced lung inflammation in cigarette smoke-exposed mice
    Oostwoud, L. C.
    Gunasinghe, P.
    Seow, H. J.
    Ye, J. M.
    Selemidis, S.
    Bozinovski, S.
    Vlahos, R.
    SCIENTIFIC REPORTS, 2016, 6
  • [3] Apocynin and ebselen reduce influenza A virus-induced lung inflammation in cigarette smoke-exposed mice
    L. C. Oostwoud
    P. Gunasinghe
    H. J. Seow
    J. M. Ye
    S. Selemidis
    S. Bozinovski
    R. Vlahos
    Scientific Reports, 6
  • [4] Mate tea reduced acute lung inflammation in mice exposed to cigarette smoke
    Lanzetti, Manuella
    Bezerra, Frank Silva
    Romana-Souza, Bruna
    Brando-Lima, Aline Cristina
    Goncalves Koatz, Vera Lucia
    Porto, Luis Cristovao
    Valenca, Samuel Santos
    NUTRITION, 2008, 24 (04) : 375 - 381
  • [5] Pulmonary inflammation in mice exposed to mainstream cigarette smoke
    Hodge-Bell, Kimberly C.
    Lee, K. Monica
    Renne, Roger A.
    Gideon, Kathy M.
    Harbo, Sam J.
    McKinney, Willie J.
    INHALATION TOXICOLOGY, 2007, 19 (04) : 361 - 376
  • [6] LUNG HYDROXYPROLINE LEVELS IN MICE EXPOSED TO CIGARETTE SMOKE
    ROSENKRANTZ, H
    ESBER, HJ
    SPRAGUE, R
    LIFE SCIENCES PART 1 PHYSIOLOGY AND PHARMACOLOGY AND PART 2 BIOCHEMISTRY GENERAL AND MOLECULAR BIOLOGY, 1969, 8 (11P1): : 571 - +
  • [7] Early Detection of Susceptibility to Acute Lung Inflammation by Molecular Imaging in Mice Exposed to Cigarette Smoke
    Perez-Rial, Sandra
    del Puerto-Nevado, Laura
    Gonzalez-Mangado, Nicolas
    Peces-Barba, German
    MOLECULAR IMAGING, 2011, 10 (05) : 398 - 405
  • [8] Impaired lung homeostasis in neonatal mice exposed to cigarette smoke
    McGrath-Morrow, Sharon
    Rangasamy, Tirumalai
    Cho, Cecilia
    Sussan, Thomas
    Neptune, Enid
    Wise, Robert
    Tuder, Rubin M.
    Biswall, Shyam
    AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2008, 38 (04) : 393 - 400
  • [9] Early and late effects of aspirin and naproxen on microRNAs in the lung and blood of mice, either unexposed or exposed to cigarette smoke
    Izzotti, Alberto
    Balansky, Roumen
    Ganchev, Gancho
    Iltcheva, Marietta
    Longobardi, Mariagrazia
    Pulliero, Alessandra
    Camoirano, Anna
    D'Agostini, Francesco
    Geretto, Marta
    Micale, Rosanna T.
    La Maestra, Sebastiano
    Miller, Mark Steven
    Steele, Vernon E.
    De Flora, Silvio
    ONCOTARGET, 2017, 8 (49) : 85716 - 85748
  • [10] Modulation by aspirin and naproxen of nucleotide alterations and tumors in the lung of mice exposed to environmental cigarette smoke since birth
    La Maestra, Sebastiano
    D'Agostini, Francesco
    Izzotti, Alberto
    Micale, Rosanna T.
    Mastracci, Luca
    Camoirano, Anna
    Balansky, Roumen
    Trosko, James E.
    Steele, Vernon E.
    De Flora, Silvio
    CARCINOGENESIS, 2015, 36 (12) : 1531 - 1538