Isolation and characterization of microsatellite markers for Pinellia ternata and cross-species amplification

被引：1
|
作者：
Chen, Fangjian [1 ]
Zhang, Lixin [2 ]
Zhao, Changming [1 ]
机构：
[1] Lanzhou Univ, Sch Life Sci, MOE Key Lab Arid & Grassland Ecol, Inst Mol Ecol, Lanzhou 730000, Peoples R China
[2] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
来源：
MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 06期
基金：
中国国家自然科学基金;
关键词：
cross-amplification; genetic structure; microsatellite; Pinellia ternata;
D O I：
10.1111/j.1755-0998.2008.02326.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, we isolated and characterized 12 microsatellite loci for Pinellia ternata. Polymorphism of these 12 loci was assessed in 46 individuals collected from two wild populations. All the loci were polymorphic with four to 13 alleles per locus and the observed and expected heterozygosities ranged from 0.312 to 0.680 and from 0.506 to 0.734, respectively. None of the loci showed significant deviation from Hardy-Weinberg equilibrium (P<0.05). No significant linkage disequilibrium was observed between pairs of studied loci. In addition, most markers amplified successfully in three closely related taxa that are Pinellia cordata, P. peltata and P. pedatisecta. These microsatellite markers could provide a useful tool for genetic structure studies of the Pinellia species.
引用
收藏
页码：1460 / 1462
页数：3
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