Expression of bovine interleukin-2 in insect cells by recombinant baculovirus

被引：1
|
作者：
Kashima, T [1 ]
Iwata, H [1 ]
Inoue, T [1 ]
机构：
[1] Yamaguchi Univ, Fac Agr, Dept Vet Hyg, Yamaguchi 7538515, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 1999年 / 61卷 / 06期
关键词：
bovine interleukin-2; insect cell; recombinant baculovirus;
D O I：
10.1292/jvms.61.705
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Bovine interleukin-2 (bIL-2) was expressed in insect cells infected with recombinant baculovirus. The cDNA for bIL-2 was amplified by reverse transcriptional polymerase chain reaction and recombinant baculovirus was constructed by homologous recombination. The recombinant baculovirus was plaque-purified, amplified, and then infected to Sf9 cells for expression of recombinant bIL-2 (rbIL-2). In result, the protein band corresponding to rbIL-2 could be detected on SDS-PAGE with coomassie brilliant blue staining and on immunoblot analysis reacted with mouse antiserum against bIL-2. In addition, cell proliferation assay for bIL-2 activity demonstrated that the culture supernatant of Sf9 cells infected with recombinant baculovirus enhanced the proliferation of bovine peripheral blood mononuclear cells in a dose-dependent manner.
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收藏
页码：705 / 707
页数：3
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