Evaluation of arboviruses of public health interest in free-living non-human primates (Alouatta spp., Callithrix spp., Sapajus spp.) in Brazil

被引:5
|
作者
da Rocha, Tatiana Carneiro [1 ]
Batista, Paulo Mira [2 ,3 ]
Andreotti, Renato [4 ]
Dalla Bona, Ana Caroline [5 ]
Navarro da Silva, Mario Antonio [5 ]
Lange, Rogerio [6 ]
Svoboda, Walfrido Kuehl [7 ]
Gomes, Eliane Carneiro [1 ]
机构
[1] UFPR, PPCF, BR-80210170 Curitiba, Parana, Brazil
[2] Secretaria Estadual Mato Grosso Sul, Coordenadoria Estadual Vigilancia Epidemiol, Campo Grande, MS, Brazil
[3] Univ Fed Mato Grosso do Sul, Programa Posgrad Doencas Infecciosas & Parasitari, Campo Grande, MS, Brazil
[4] Empresa Brasileira Pesquisa Agr, Embrapa Gado Corte, Campo Grande, MS, Brazil
[5] Univ Fed Parana, Dept Zool, Setor Ciencias Biol, BR-80060000 Curitiba, Parana, Brazil
[6] Univ Fed Parana, Dept Vet Med, Setor Ciencias Biol, BR-80060000 Curitiba, Parana, Brazil
[7] Univ Fed Integracao Latinoamer, Inst Latinoamer Ciencias Vida & Nat, Foz Do Iguacu, Parana, Brazil
关键词
Non-human primates; Arbovirus; RT-PCR; Zoonosis; MATO-GROSSO; CARAYA; SUL;
D O I
10.1590/0037-8682-0024-2015
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Introduction: The aim of the present study was to evaluate the presence of arboviruses from the Flavivirus genus in asymptomatic free-living non-human primates (NHPs) living in close contact with humans and vectors in the States of Parana and Mato Grosso do Sul, Brazil. Methods: NHP sera samples (total n = 80, Alouatta spp. n = 07, Callithrix spp. n = 29 and Sapajus spp. n = 44) were screened for the presence of viral genomes using reverse transcription polymerase chain reaction and 10% polyacrylamide gel electrophoresis techniques. Results: All of the samples were negative for the Flavivirus genome following the 10% polyacrylamide gel electrophoresis analysis. Conclusions: These negative results indicate that the analyzed animals were not infected with arboviruses from the Flavivirus genus and did not represent a risk for viral transmission through vectors during the period in which the samples were collected.
引用
收藏
页码:143 / 148
页数:6
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