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Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation
被引:93
|作者:
Winchell, Jonas M.
[1
]
Thurman, Kathleen A.
[1
]
Mitchell, Stephanie L.
[1
]
Thacker, W. Lanier
[1
]
Fields, Barry S.
[1
]
机构:
[1] Ctr Dis Control & Prevent, Resp Dis Branch, Atlanta, GA 30333 USA
关键词:
D O I:
10.1128/JCM.00440-08
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.
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页码:3116 / 3118
页数:3
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