Metal-containing proteins were detected directly in separated protein bands in one-dimensional gels, by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) as an atomic mass spectrometric technique. In order to study the binding of Cu and Zn on tau protein isoforms as a target protein in Alzheimer's disease, enriched isotope tracers (Cu-65 and Zn-67) were doped to one-dimensional gels of separated tau protein isoforms after gel electrophoresis. In several protein bands metal ions were detected and Cu-65/Cu-63 and Zn-67/Zn-64 isotope ratios were measured by LA-ICP-MS. The isotope analysis by LA-ICP-MS indicates certain proteins with a natural isotope composition of Cu or Zn. However, copper-containing tau protein isoforms with a changed isotope ratio in comparison to the isotope composition in nature were also found. This experimental finding demonstrates the formation of new metal-containing tau protein complexes during the tracer experiments in the 1-D gel. We attempted to identify the protein bands from tau proteins separated by one-dimensional (1-D) gel electrophoresis by using biopolymer mass spectrometry with MALDI-FTICR-MS, after excision from the 1-D gel and tryptic digestion.
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RAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, RussiaRAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, Russia
Novoselov, Konstantin
Belogub, Elena
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RAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, RussiaRAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, Russia
Belogub, Elena
Shilovskikh, Vladimir
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St Petersburg State Univ, Ctr Geoenvironm Res & Modeling GEOMODEL, St Petersburg, RussiaRAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, Russia
Shilovskikh, Vladimir
Artemyev, Dmitry
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RAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, RussiaRAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, Russia
Artemyev, Dmitry
Blinov, Ivan
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RAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, RussiaRAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, Russia
Blinov, Ivan
Filippova, Ksenia
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RAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, RussiaRAS, Inst Mineral, South Urals Fed Res Ctr Mineral & Geoecol UB, Miass, Russia
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Hampshire Coll, Sch Nat Sci, Amherst, MA 01002 USAHampshire Coll, Sch Nat Sci, Amherst, MA 01002 USA
Alexander, David
Ellerby, Ryan
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Hampshire Coll, Sch Nat Sci, Amherst, MA 01002 USAHampshire Coll, Sch Nat Sci, Amherst, MA 01002 USA
Ellerby, Ryan
Hernandez, Avery
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Hampshire Coll, Sch Nat Sci, Amherst, MA 01002 USAHampshire Coll, Sch Nat Sci, Amherst, MA 01002 USA
Hernandez, Avery
Wu, Fengchang
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Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R ChinaHampshire Coll, Sch Nat Sci, Amherst, MA 01002 USA
Wu, Fengchang
Amarasiriwardena, Dulasiri
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Hampshire Coll, Sch Nat Sci, Amherst, MA 01002 USAHampshire Coll, Sch Nat Sci, Amherst, MA 01002 USA