Quadruplex DNA-Stabilising Dinuclear Platinum(II) Terpyridine Complexes with Flexible Linkers

被引：28
|
作者：
Ang, Dale L. [1 ]
Harper, Benjamin W. J. [1 ]
Cubo, Leticia [2 ]
Mendoza, Oscar [2 ]
Vilar, Ramon [2 ,3 ]
Aldrich-Wright, Janice [1 ]
机构：
[1] Univ Western Sydney, Nanoscale Org & Dynam Grp, Sch Sci & Hlth, Penrith, NSW 2751, Australia
[2] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England
[3] Univ London Imperial Coll Sci Technol & Med, Inst Chem Biol, London SW7 2AZ, England
来源：
CHEMISTRY-A EUROPEAN JOURNAL | 2016年 / 22卷 / 07期
基金：
英国工程与自然科学研究理事会;
关键词：
circular dichroism; DNA; FRET; G-quadruplexes; platinum; RADIATION CIRCULAR-DICHROISM; TELOMERIC G-QUADRUPLEX; SYNCHROTRON-RADIATION; METAL-COMPLEXES; STRUCTURAL BASIS; SPECTROSCOPY; MYC; STANDARDIZATION; VISUALIZATION; POLYMORPHISM;
D O I：
10.1002/chem.201503663
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Four dinuclear terpyridineplatinum(II) (Pt-terpy) complexes were investigated for interactions with G-quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 degrees C and maintain this in up to a 600-fold excess of dsDNA. This study demonstrates that dinuclear Pt-terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA.
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页码：2317 / 2325
页数：9
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