Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium majus L. and Berberis thunbergii DC. toward Hematopoietic Cancer Cell Lines

被引：40
|
作者：
Och, Anna [1 ]
Zalewski, Daniel [1 ]
Komsta, Lukasz [2 ]
Kolodziej, Przemyslaw [1 ]
Kocki, Janusz [3 ]
Bogucka-Kocka, Anna [1 ]
机构：
[1] Med Univ Lublin, Chair & Dept Biol & Genet, 4a Chodzki St, PL-20093 Lublin, Poland
[2] Med Univ Lublin, Chair & Dept Med Chem, 4 Jaczewskiego St, PL-20090 Lublin, Poland
[3] Med Univ Lublin, Dept Clin Genet, Chair Med Genet, 11 Radziwillowska St, PL-20080 Lublin, Poland
来源：
TOXINS | 2019年 / 11卷 / 09期
关键词：
cytotoxicity; apoptosis; sanguinarine; berberine; Chelidonium majus; Berberis thunbergii; leukemia; anticancer; PRIMARY EFFUSION LYMPHOMA; BCL-2 FAMILY PROTEINS; ALKALOID SANGUINARINE; INDUCED APOPTOSIS; GENE-EXPRESSION; DNA; BENZOPHENANTHRIDINE; INHIBITION; INDUCTION; ACETYLCHOLINESTERASE;
D O I：
10.3390/toxins11090485
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Isoquinoline alkaloids belong to the toxic secondary metabolites occurring in plants of many families. The high biological activity makes these compounds promising agents for use in medicine, particularly as anticancer drugs. The aim of our study was to evaluate the cytotoxicity and proapoptotic activity of sanguinarine, berberine, and extracts of Chelidonium majus L. and Berberis thunbergii DC. IC10, IC50, and IC90 doses were established toward hematopoietic cancer cell lines using trypan blue staining. Alterations in the expression of 18 apoptosis-related genes in cells exposed to IC10, IC50, and IC90 were evaluated using real-time PCR. Sanguinarine and Chelidonium majus L. extract exhibit significant cytotoxicity against all studied cell lines. Lower cytotoxic activity was demonstrated for berberine. Berberis thunbergii DC. extract had no influence on cell viability. Berberine, sanguinarine, and Chelidonium majus L. extract altered the expression of apoptosis-related genes in all tested cell lines, indicating the induction of apoptosis. The presented study confirmed the substantial cytotoxicity and proapoptotic activity of sanguinarine, berberine, and Chelidonium majus L. extract toward the studied hematopoietic cell lines, which indicates the utility of these substances in anticancer therapy.
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页数：19
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