M.BstF5I-4, the forth DNA-methyltransferase of the BstF5I restriction-modification system from Bacillus stearothermophilus F5

被引：0
|
作者：
Golikova, LN [1 ]
Gutorov, VV
Evdokimov, AA
Shchelkunov, SN
Gonchar, DA
Okhapkina, SS
Degtyarev, SK
Netesova, NA
机构：
[1] Vector State Res Ctr Virol & Biotechnol, Inst Mol Biol, Koltsov 630559, Novosibirsk Obl, Russia
[2] Russian Acad Sci, Inst Mol Biol & Biophys, Siberian Div, Novosibirsk 630117, Russia
[3] SibEnzyme, Novosibirsk 630117, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2002年 / 28卷 / 01期
基金：
俄罗斯基础研究基金会;
关键词：
Bacillus stearothermophilus; DNA-methyltransferase; restriction-modification systems;
D O I：
10.1023/A:1013966522353
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The fourth DNA-methyltransferase of the BstF5I restriction-modification (RM) system from Bacillus stearothermophilus F5 (M.BstF5I-4) was discovered, which modifies the adenine residue within the upper strand of the recognition site 5'-GGATG-3'/5'-CATCC-3'. Thus, unlike other known RM systems, the BstF5I RM system comprises four genes encoding DNA-methyltransferases, three of which possess the same substrate specificity and methylate adenine within the 5'-GGATG sequence.
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收藏
页码：77 / 79
页数：3
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