A simple and reversible fluorescent probe based on NBD for rapid detection of hypochlorite and its application for bioimaging

被引：27

作者：

Shen, Youming ^{[1
,2
]}

Zhang, Xiangyang ^{[1
]}

Huang, Xi ^{[2
]}

Wen, Siyu ^{[1
]}

Liu, Mingdi ^{[1
]}

Deng, Yan ^{[1
]}

Zhang, Youyu ^{[2
]}

Zhang, Chunxiang ^{[1
]}

Jin, Junling ^{[1
]}

Li, Haitao ^{[2
]}

Yao, Shouzhuo ^{[2
]}

机构：

[1] Hunan Univ Arts & Sci, Coll Chem & Chem Engn, Changde 415000, Peoples R China

[2] Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China

来源：

RSC ADVANCES | 2015年 / 5卷 / 97期

基金：

中国国家自然科学基金;

关键词：

HIGHLY SELECTIVE DETECTION; REACTIVE OXYGEN; REDOX CYCLE; OXIDATIVE STRESS; SE-BODIPY; IN-VITRO; ACID; MYELOPEROXIDASE; SENSOR; FLUOROPHORE;

D O I：

10.1039/c5ra15373e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple and reversible fluorescent probe bearing 7-nitrobenz-2-oxa-1,3-diazole and a selenomorpholine fragment was designed and synthesized. The probe showed highly selective, sensitive and fast (<10 s) recognition to hypochlorite in aqueous solutions. The relative results demonstrated that the linear response range of the probe was between 5.0 x 10(-8) M and 1.2 x 10(-4) M, with a low detection limit of 3.3 nM (S/N = 3). The probe was capable of monitoring hypochlorite reversibly in the presence of glutathione. In addition, the biological applications in living cells have been described.

引用

页码：79519 / 79524

页数：6

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