Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites

被引:10
|
作者
Moreirara, Fatima M. S. [1 ]
Lange, Anderson [2 ]
Klauberg-Filho, Osmar [3 ]
Siqueira, Jose O. [1 ]
Nobrega, Rafaela S. A. [1 ]
Lima, Adriana S. [1 ]
机构
[1] Univ Fed Lavras UFLA, DCS, Programa Posgrad Solos & Nutricao Plantas, BR-37200000 Lavras, MG, Brazil
[2] UNEMAT, Dept Agron, BR-78580000 Alta Floresta, MT, Brazil
[3] Univ Estado Santa Catarina UDESC, BR-88520000 Lages, SC, Brazil
来源
关键词
Azospirillum; Zinc; Cadmium; soil contaminants; Nitrogen-fixing bacteria;
D O I
10.1590/S0001-37652008000400014
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
This work aimed to evaluate density of associative diazotrophic bacteria populations in soil and grass root samples from heavy metal contaminated sites, and to characterize isolates from these populations, both, phenotypically (Zinc, Cadmium and NaCl tolerance in vitro, and protein profiles) and genotypically (16S rDNA sequencing), as compared to type strains of known diazotrophic species. Densities were evaluated by using NFb, Fam and JNFb media, commonly used for enrichment cultures of diazotrophic bacteria. Bacterial densities found in soil and grass root samples from contaminated sites were similar to those reported for agricultural soils. Azospirillum spp. isolates from contaminated sites and type strains from non-contaminated sites varied substantially in their in vitro tolerance to Zn+2 and Cd+2, being Cd+2 more toxic than Zn+2. Among the most tolerant isolates (UFLA 1S, 1R, S181, S34 and S22), some (1R, S34 and S22) were more tolerant to heavy metals than rhizobia from tropical and temperate soils. The majority of the isolates tolerant to heavy metals were also tolerant to salt stress as indicated by their ability to grow in solid medium supplemented with 30 g L-1 NaCl. Five isolates exhibited high dissimilarity in protein profiles, and the 16S rDNA sequence analysis of two of them revealed new sequences for Azospirillum.
引用
收藏
页码:749 / 761
页数:13
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