Acylated Iridoids from the Roots of Valeriana officinalis var. latifolia

Phytochemical investigation of the roots of Valeriana officinalis var. latifolia resulted in the isolation and characterization of six new acylated iridoids, (5S,7S,8S,9S)-7-hydroxy-8-isovaleroyloxy Delta(4,11)-dihyronepetalactone (1),(5S,7S,8S,9S)-7-hydroxy-10-isovaleroyloxy-Delta(4,11)-dihyronepetalactone (2),(5S,8S,9S)-10-isovaleroyloxy-Delta(4,11)-dihyronepetalactone (3), (5S, 6S, 8S, 9R)-6-isovaleroyloxy-Delta(4,11)-1,3-diol (4), (5S, 6S, 8S, 9R)-1,3-isovaleroxy-Delta(4,11)-1,3-diol (5), and (5S, 6S, 8S, 9R)-3-isovaleroxy- 6-isovaleroyloxy-Delta(4,11)-1,3-diol (6). Their structures were determined mainly by 1D and 2D NMR spectroscopic techniques. We also report herein for the first time the single crystal X-ray structure of compound 1. In addition, the cytotoxic activities of compounds 1-6 were evaluated against A549 (human lung adenocarcinoma), HCT116 (human colon carcinoma), SK-BR-3 (human breast carcinoma), and HepG2 (human hepatoma) cell lines. Compound 6 showed weak cell growth inhibition of A549, HCT116, SK-BR-3, and HepG2 cells.