An Amperometric Glucose Biosensor based on Pd-Pt Nanodendrites and Chitosan Matrix

We report on the utilization of Pd-Pt nanodendrites (Pd-Pt NDs) to create a highly responsive glucose biosensor. Glucose oxidase was simply mixed with Pd-Pt NDs and cross-linked on the Pt electrode with chitosan (CHIT) medium by glutaraldehyde. The biosensor showed low linear detection limit (0.6 mu M), wide linear detection range (0.001-5 mM). The excellent performance of the biosensor is attributed to relatively large surface areas and particularly catalytic active of Pd-Pt NDs, and good biocompatibility of CHIT, which enhances the enzyme absorption and promotes electron transfer between redox enzymes and the surface of electrodes. This composite film can be easily extended to immobilize other biomolecules.