Systematic evaluation of phenolic compounds and protective capacity of a new mulberry cultivar J33 against palmitic acid-induced lipotoxicity using a simulated digestion method

被引：69

作者：

Hu, Dongwen ^{[1
]}

Xu, Yang ^{[1
]}

Xie, Jiahong ^{[1
]}

Sun, Chongde ^{[2
]}

Zheng, Xiaodong ^{[1
]}

Chen, Wei ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Food Sci & Nutr,Zhejiang Key Lab Agrofood Pr, Natl Engn Lab Intelligent Food Technol & Equipmen, Key Lab Agroprod Postharvest Handling,Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, State Agr Minist, Lab Fruit Qual Biol,Lab Hort Plant Growth Dev & Q, Hangzhou 310058, Zhejiang, Peoples R China

来源：

FOOD CHEMISTRY | 2018年 / 258卷

基金：

中国国家自然科学基金;

关键词：

Mulberry; Palmitic acid; Lipotoxicity; Oxidative stress; Triglycerides synthesis; Simulated digestion; SATURATED FATTY-ACIDS; MEDIATED LIPOTOXICITY; MOLECULAR-MECHANISMS; AFFORDS PROTECTION; HEPATIC STEATOSIS; LIVER-DISEASE; DNA-DAMAGE; MORUS-ALBA; EXTRACT; PHYTOCHEMICALS;

D O I：

10.1016/j.foodchem.2018.03.049

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This research aimed to investigate the protective effects of a new mulberry cultivar J33 with simulated gastrointestinal digestion against palmitic acid (PA)-induced lipotoxicity. LC-MS analysis revealed that the contents of four flavonoid glycosides (quercetin rhamnosylhexoside hexoside, quercetin rhamnosylhexoside, quercetin hexoside, kaempferol rhamnosylhexoside) increased after digestion. Besides, mulberry digest (MBD) at 0.5-2 mg/mL significantly reduced PA-induced lipotoxicity in human hepatocytes, while mulberry extract without digestion (MBE) showed no protection. Further investigations demonstrated that the protection of MBD was attributed to two aspects. On the one hand, MBD could attenuate PA-induced oxidative stress by suppressing ROS accumulation, regulating intracellular glutathione and ameliorating mitochondrial dysfunction. On the other hand, MBD could promote PA incorporation into inert triglycerides (TG) to deal with the acute lipid overload, reducing the lipotoxicity caused by PA. Overall, our research might provide a new perspective of mulberry cultivar J33 in ameliorating non-alcoholic fatty liver disease (NAFLD).

引用

页码：43 / 50

页数：8

共 1 条

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Xia, Ling-Li
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