Plasmonic Photothermal Fluorescence Modulation for Homogeneous Biosensing

被引:19
|
作者
Pellegrotti, Jesica V. [1 ]
Cortes, Emiliano [1 ]
Bordenave, Martin D. [1 ]
Caldarola, Martin [2 ]
Kreuzer, Mark P. [1 ]
Sanchez, Alfredo D. [1 ]
Ojea, Ignacio [3 ,4 ]
Bragas, Andrea V. [2 ]
Stefani, Fernando D. [1 ,2 ,5 ]
机构
[1] Consejo Nacl Invest Cient & Tecn, Ctr Invest Bionanociencas CIBION, Godoy Cruz 2390,C142SFQD, Buenos Aires, DF, Argentina
[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, CONICET, IMAS,Dept Fis, Pabellon 1 Ciudad Univ,CI428EHA, Buenos Aires, DF, Argentina
[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, CONICET, IMAS,Dept Matemat, Pabellon 1 Ciudad Univ,CI428EHA, Buenos Aires, DF, Argentina
[4] Univ Buenos Aires, Fac Ciencias Exactas & Nat, CONICET, IMAS,Inst Invest Matemat Luis A Santalo, Pabellon 1 Ciudad Univ,CI428EHA, Buenos Aires, DF, Argentina
[5] ORCID, Bethesda, MD 20817 USA
来源
ACS SENSORS | 2016年 / 1卷 / 11期
关键词
plasmonic heating; gold nanorods; gold nanoparticle; fluorescence quenching; sandwich assay; METAL NANOPARTICLES; IMAGING MICROSCOPY; OPTICAL-PROPERTIES; TEMPERATURE; DYES; FLUOROPHORES; ENHANCEMENT; GENERATION; RECOVERY; THERAPY;
D O I
10.1021/acssensors.6b00512
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Fluorescence readout is uniquely powerful for biological assays and imaging because it combines the detection of specific biotargets with high spatial and temporal resolution. Recently, several strategies for the modulation in time of fluorescence emission have been proven useful to separate the target signal from constant background contributions. Here, we investigate the emission modulation of organic fluorophores located in the nanometric vicinity of plasmonically heated gold nanorods and apply it to a novel, all-optical homogeneous biosensing scheme. The combination of plasmonic heating and temperature sensitive molecular fluorescence enables the robust quantification of surface reactions.
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页码:1351 / 1357
页数:7
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