Characterization of hippocampal theta rhythm in wild-type mice and glutamate receptor subunit δ2 mutant mice during eyeblink conditioning with a short trace interval

We have shown that glutamate receptor subunit 62 (GluR delta 2) null mutant mice, which have serious morphological and functional deficiencies in the cerebellar cortex, are severely impaired in delay eyeblink conditioning but not in trace eyeblink conditioning, even with a 0-trace interval. Such 0-trace conditioning does not depend critically on the hippocampus in wild-type mice, but it does in GluR delta 2 mutant mice. Here we examined the hippocampal electroencephalogram (EEG) during 0-trace conditioning in GluR delta 2 mutant and wild-type mice. During the apparatus habituation sessions, the total hippocampal theta activity (4-12 Hz) of GluR delta 2 mutant mice was less than that of wildtype mice. Activity in the higher frequency band (8-12 Hz, type 1) in GluR delta 2 mutant mice was significantly less than it was in wild-type mice, but activity in the lower frequency band (4-8 Hz, type 2) was not. As learning proceeded during the acquisition sessions, the total theta activity decreased in many of the wild-type mice, while this phenomenon was less prominent in GluR delta 2 mutant mice. Further analysis showed that the type 1 activity in wild-type mice increased in the early sessions and then decreased, while that in GluR delta 2 mutant mice did not change. Type 2 activity tended to decrease in both types of mice as the conditioning proceeded. These results indicate that the distribution of hippocampal EEG frequency and its properties during conditioning are different between wild-type and GluR delta 2 mutant mice, suggesting that the cerebellar cortical dysfunction may cause an alteration in the electrophysiological characteristics of the hippocampus. (C) 2005 Elsevier B.V. All rights reserved.