Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses

被引：55

作者：

Chan, Jasper Fuk-Woo ^{[1
,2
,3
,4
]}

Choi, Garnet Kwan-Yue ^{[2
]}

Tsang, Alan Ka-Lun ^{[2
]}

Tee, Kah-Meng ^{[2
]}

Lam, Ho-Yin ^{[5
]}

Yip, Cyril Chik-Yan ^{[2
]}

To, Kelvin Kai-Wang ^{[1
,2
,3
,4
]}

Cheng, Vincent Chi-Chung ^{[2
]}

Yeung, Man-Lung ^{[2
]}

Lau, Susanna Kar-Pui ^{[1
,2
,3
,4
]}

Woo, Patrick Chiu-Yat ^{[1
,2
,3
,4
]}

Chan, Kwok-Hung ^{[2
]}

Tang, Bone Siu-Fai ^{[5
]}

Yuen, Kwok-Yung ^{[1
,2
,3
,4
]}

机构：

[1] Univ Hong Kong, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China

[2] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

[3] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China

[4] Univ Hong Kong, Carol Yu Ctr Infect, Hong Kong, Hong Kong, Peoples R China

[5] Hong Kong Sanat & Hosp, Dept Pathol, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 08期

关键词：

RESPIRATORY SYNDROME CORONAVIRUS; CELL-LINE; HCOV-EMC; VIRUS; DISEASE; TESTS; LNA; AMPLIFICATION; PERFORMANCE; VALIDATION;

D O I：

10.1128/JCM.01224-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.

引用

页码：2722 / 2726

页数：5

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