Genomic resource development for shellfish of conservation concern

被引:12
|
作者
Timmins-Schiffman, Emma B. [1 ]
Friedman, Carolyn S. [1 ]
Metzger, Dave C. [1 ]
White, Samuel J. [1 ]
Roberts, Steven B. [1 ]
机构
[1] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA
关键词
gene expression; high-throughput sequencing; Olympia oyster; pinto abalone; transcriptome; LURIDA CARPENTER 1864; KAMTSCHATKANA-KAMTSCHATKANA ABUNDANCE; SAN-JUAN ARCHIPELAGO; HEAT-SHOCK PROTEINS; GENE-EXPRESSION; HALIOTIS-KAMTSCHATKANA; OLYMPIA OYSTER; ABALONE POPULATIONS; SIGNALING PATHWAYS; BRITISH-COLUMBIA;
D O I
10.1111/1755-0998.12052
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Effective conservation of threatened species depends on the ability to assess organism physiology and population demography. To develop genomic resources to better understand the dynamics of two ecologically vulnerable species in the Pacific Northwest of the United States, larval transcriptomes were sequenced for the pinto abalone, Haliotis kamtschatkana kamtschatkana, and the Olympia oyster, Ostrea lurida. Based on comparative species analysis the Ostrea lurida transcriptome (41136 contigs) is relatively complete. These transcriptomes represent the first significant contribution to genomic resources for both species. Genes are described based on biological function with particular attention to those associated with temperature change, oxidative stress and immune function. In addition, transcriptome-derived genetic markers are provided. Together, these resources provide valuable tools for future studies aimed at conservation of Haliotis kamtschatkana kamtschatkana, Ostrea lurida and related species.
引用
收藏
页码:295 / 305
页数:11
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