Tryptophan 232 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences rearrangement and deprotonation but not cyclization reactions

被引：28
|
作者：
Wu, TK [1 ]
Yu, MT
Liu, YT
Chang, CH
Wang, HJ
Diau, EWG
机构：
[1] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu 300, Taiwan
[2] Natl Chiao Tung Univ, Dept Appl Chem, Hsinchu 300, Taiwan
来源：
ORGANIC LETTERS | 2006年 / 8卷 / 07期
关键词：
D O I：
10.1021/ol053134w
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Oxidosqualene-lanosterol cyclases convert oxidosqualene to lanosterol in yeast and mammals. Site-saturated mutants' construction of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase, at Trp232 exchanges against proteinogenic amino acids, and product profiles are shown. All mutants, except Lys and Arg, produced protosta-12,24-dien-3 beta-ol, lanosterol, and parkeol. Overall, Trp232 plays a catalytic role in the influence of rearrangement process and determination of deprotonation position but does not involve intervention in the cyclization steps.
引用
收藏
页码：1319 / 1322
页数：4
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