Isolation and characterization of 14 polymorphic microsatellite DNA loci for the endangered Whooping Crane (Grus americana) and their applicability to other crane species (vol 2, pg 251, 2010)

被引:1
|
作者
Jones, Kenneth L. [1 ,2 ,5 ]
Henkel, Jessica R. [3 ,4 ]
Howard, Jerome J. [4 ]
Lance, Stacey L. [5 ]
Hagen, Chris [5 ]
Glenn, Travis C. [1 ,2 ,5 ]
机构
[1] Univ Georgia, Georgia Genom Facil, Athens, GA 30602 USA
[2] Univ Georgia, Dept Environm Hlth Sci, Athens, GA 30602 USA
[3] Tulane Univ, Dept Ecol & Evolutionary Biol, New Orleans, LA 70118 USA
[4] Univ New Orleans, Dept Biol Sci, New Orleans, LA 70148 USA
[5] Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA
关键词
Cranes; Grus americana; Grus canadensis; Microsatellite DNA; PCR primers;
D O I
10.1007/s12686-010-9250-1
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Fourteen microsatellite DNA loci were isolated from the endangered Whooping Crane (Grus americana) and genetic variability assessed from 45 captive reared individuals. Allele numbers detected at each locus ranged from 2 to 6, the highest seen for this species. Mean observed heterozygosity varied from 0.04 to 0.79. These markers were then successfully amplified for two non-migratory populations of Sandhill Crane [Florida (Grus canadensis pratensis) and Missisippi (Grus canadensis pulla)], underscoring their utility for the conservation of threatened crane species.
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页码:255 / 255
页数:1
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