Objective: It was aimed to investigate the knowledge, attitudes and behaviors of health professionals such as specialist doctors, assistant doctors, and nurses in charge of COVID-19 vaccination about adrenaline application.Material and Methods: With the questionnaire method, the occupa-tional groups, demographic characteristics, knowledge, education and experience levels of the participants were investigated.Results: Of the 218 participants included in the study, there were 15 (6.9%) specialists, 107 (49.1%) assistant, 2 (0.9%) practitioners, and 94 (43.1%) nurses. It was observed that 78.9% of the participants preferred adrenaline as the first drug to be administered in the treatment of ana-phylaxis. Among the participants who said adrenaline is the first drug to be administered in the treatment of anaphylaxis, 97 were (90.7%) as-sistants, 59 (62.8%) were nurses, 14 (93.3%) were specialist doctors and 2 (90.7%) 100) were practitioners (p= 0.002). Of the 165 (75.7%) partic-ipants who reported that the vastus lateralis muscle is the right place for intramuscular adrenaline administration, 57 (60.6%) were nurses, 97 (90.7%) were assistant doctors, 10 (66.7%) were specialist doctors and 1 (50%) was a practitioner (p< 0.001). It was determined that 143 (65.6%) of the participants had received prior training on adrenaline administra-tion. It was determined that 106 (74.1%) of the healthcare professionals who had received adrenaline training and 39 (52%) of those who had not been trained chose intramuscular administration as the mode of admin-istration of adrenaline during anaphylaxis (p= 0.001).Conclusion: In our study, it was determined that most of the health pro-fessionals had received training on the use of adrenaline. While these re-sults are good, they are not sufficient. For this reason, all health personnel should be educated on anaphylaxis diagnosis and the use of adrenaline.