Exploring genetic polymorphisms and transcript levels of antioxidant and metabolic markers for prediction and monitoring diarrhea in Holstein dairy calves

被引：2

作者：

Ateya, Ahmed ^{[1
]}

Al-Sharif, Mona ^{[2
]}

Faraj, Salah H. ^{[3
]}

Abdo, Mohamed ^{[4
,5
]}

Fericean, Liana ^{[6
]}

Banatean-Dunea, Ioan ^{[6
]}

Hammad, Salam J. ^{[7
]}

Mamdouh, Maha ^{[8
]}

Fayed, Hayat ^{[9
]}

Marawan, Marawan A. ^{[10
]}

Raslan, Walaa S. ^{[8
]}

机构：

[1] Mansoura Univ, Fac Vet Med, Dept Dev Anim Wealth, Mansoura, Egypt

[2] Univ Jeddah, Coll Sci, Dept Biol, Jeddah, Saudi Arabia

[3] Univ Misan, Coll Sci, Dept Biol, Maysan, Iraq

[4] Badr Univ Cairo BUC, Sch Vet Med, Dept Anim Histol & Anat, Cairo, Egypt

[5] Univ Sadat City, Fac Vet Med, Dept Anat & Embryol, Sadat City, Egypt

[6] Univ Life Sci King Michael I, Fac Agr Sci, Dept Biol & Plant Protect, Timisoara, Romania

[7] Natl Univ Sci & Technol, Coll Hlth & Med Technol, Dept Med Lab Tech, Dhi Qar, Iraq

[8] Benha Univ, Fac Vet Med, Dept Physiol, Toukh, Egypt

[9] Benha Univ, Fac Vet Med, Dept Anim Med Internal Med, Banha, Egypt

[10] Benha Univ, Fac Vet Med, Dept Anim Med Infect Dis, Banha, Egypt

来源：

ACTA AGRICULTURAE SCANDINAVICA SECTION A-ANIMAL SCIENCE | 2023年

关键词：

Dairy calves; genetic polymorphisms; transcript level; diarrhea; GENOME-WIDE ASSOCIATION; OXIDATIVE STRESS; CALF DIARRHEA; HEALTH TRAITS; RISK-FACTORS; VARIANTS; PREVALENCE; PATHOGENESIS; EXPRESSION; MORBIDITY;

D O I：

10.1080/09064702.2023.2274881

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The objective of this study was to investigate the genetic polymorphisms and transcript levels of antioxidant and metabolic markers for diarrhea monitoring in Holstein dairy calves. For DNA and RNA extraction, blood samples were taken from 100 female calves (50 with diarrhea and 50 appearing healthy). DNA sequencing and real time PCR for and metabolic genes were performed to identify genetic variants linked to diarrhea susceptibility. Results revealed that there were nucleotide sequence variations in the investigated genes between healthy and diarrheal calves. Keap1, HMOX1, CMPK2, ASPG, FPGT, TNNI3 K, and LPCAT1 were considerably more highly expressed in diarrheic calves than resistant ones. The Nrf2, PRDX2, and PRDX6 genes, however, produced a different pattern. This study provides a practical control strategy to lessen newborn diarrhea in Holstein dairy calves and emphasizes the significance of nucleotide variations along with antioxidant and metabolic gene expression patterns as proxy markers of diarrhea susceptibility/resistance.

引用

页数：11

共 3 条

[1] The Effects of Zinc Supplementation on Growth, Diarrhea, Antioxidant Capacity, and Immune Function in Holstein Dairy Calves
Liu, Junhao
Ma, Fengtao
Degen, Allan
Sun, Peng
[J]. ANIMALS, 2023, 13 (15):
[2] Combining genetic markers, on-farm information and infrared data for the in-line prediction of blood biomarkers of metabolic disorders in Holstein cattle
Mota, Lucio F. M.
Giannuzzi, Diana
Pegolo, Sara
Toledo-Alvarado, Hugo
Schiavon, Stefano
Gallo, Luigi
Trevisi, Erminio
Arazi, Alon
Katz, Gil
Rosa, Guilherme J. M.
Cecchinato, Alessio
[J]. JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, 2024, 15 (01)
[3] Combining genetic markers,on-farm information and infrared data for the in-line prediction of blood biomarkers of metabolic disorders in Holstein cattle
Lucio FMMota
Diana Giannuzzi
Sara Pegolo
Hugo ToledoAlvarado
Stefano Schiavon
Luigi Gallo
Erminio Trevisi
Alon Arazi
Gil Katz
Guilherme JMRosa
Alessio Cecchinato
[J]. Journal of Animal Science and Biotechnology, 2024, 15 (06) : 2229 - 2241

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