Genetic and technological diversity of Streptococcus thermophilus isolated from the Saint-Nectaire PDO cheese-producing area

被引:2
|
作者
Grizon, Anna [1 ]
Theil, Sebastien [1 ]
Callon, Cecile [1 ]
Gerber, Pauline [2 ]
Helinck, Sandra [3 ]
Dugat-Bony, Eric [3 ]
Bonnarme, Pascal [3 ]
Chassard, Christophe [1 ]
机构
[1] Univ Clermont Auvergne, Fromage UMR545, INRAE, VetAgro Sup, Aurillac, France
[2] Pole Fromager AOP Mass Cent, Aurillac, France
[3] Univ Paris Saclay, INRAE, AgroParisTech, UMR SayFood, Palaiseau, France
关键词
Streptococcus thermophilus; diversity; genome comparison; functional properties; autochthonous starter; cheese; LACTIC-ACID BACTERIA; STARTER CULTURES; DAIRY; STRAINS; MILK; SAFETY; VARIABILITY; PROTEOLYSIS; GALACTOSE; SELECTION;
D O I
10.3389/fmicb.2023.1245510
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Streptococcus thermophilus is of major importance for cheese manufacturing to ensure rapid acidification; however, studies indicate that intensive use of commercial strains leads to the loss of typical characteristics of the products. To strengthen the link between the product and its geographical area and improve the sensory qualities of cheeses, cheese-producing protected designations of origin (PDO) are increasingly interested in the development of specific autochthonous starter cultures. The present study is therefore investigating the genetic and functional diversity of S. thermophilus strains isolated from a local cheese-producing PDO area. Putative S. thermophilus isolates were isolated and identified from milk collected in the Saint-Nectaire cheese-producing PDO area and from commercial starters. Whole genomes of isolates were sequenced, and a comparative analysis based on their pan-genome was carried out. Important functional properties were studied, including acidifying and proteolytic activities. Twenty-two isolates representative of the diversity of the geographical area and four commercial strains were selected for comparison. The resulting phylogenetic trees do not correspond to the geographical distribution of isolates. The clustering based on the pan-genome analysis indicates that isolates are divided into five distinct groups. A Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation of the accessory genes indicates that the accessory gene contents of isolates are involved in different functional categories. High variability in acidifying activities and less diversity in proteolytic activities were also observed. These results indicate that high genetic and functional variabilities of the species S. thermophilus may arise from a small (1,800 km2) geographical area and may be exploited to meet demand for use as autochthonous starters.
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页数:14
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