Multigenerational exposure of cadmium trans-generationally impairs locomotive and chemotactic behaviors in Caenorhabditis elegans

Cadmium is a naturally existing heavy metal and a notorious environmental pollutant. While its toxic outcomes and underlying mechanisms remain largely elusive. To explore the behavioral change caused by multigenera-tional exposure of cadmium to C. elegans, we challenged the C. elegans with cadmium for six generations and observed its impact on animal behaviors. Wild-type worms were randomly divided into two groups, the control and cadmium exposure groups. Locomotive and chemotactic behaviors were observed across six generations. Head thrashing frequency, chemotaxis index, and fold change index were used to evaluate the neurotoxicity of multigenerational cadmium exposure. Multigenerational cadmium exposure can trans-generationally increase the head thrashing frequency of C. elegans during swimming, and impair the chemotactic behaviors to isoamyl alcohol, diacetyl, and 2-nonanone. Our findings proposed a trans-generationally behavioral impact induced by multigenerational cadmium exposure.