High-resolution magic-angle spinning NMR metabolic profiling with spatially localized spectroscopy under slow sample spinning

Owing to its feasibility and versatility, High-Resolution Magic-Angle Spinning (HRMAS) NMR spectroscopy is considered an essential analytical technique in metabolomics for assessing the biochemical composition of tissue samples. Localized profiling with HRMAS has recently emerged and shown promise for spatial resolution of metabolic profiles within the sampling tissues. However, the requisite sample spinning in a few kHz can perturb the tissues spatially and morphologically. This study explored a simple approach to slow sample spinning experiments at 500 Hz without needing pulse-assist sideband suppression experiments to acquire localized spectral data. Slow-spinning localized one-and two-dimensional spectroscopy, including Total Correlation Spectroscopy (TOCSY), were explored on soft tissues for metabolic profiling. We also examined inhomogeneous radiofrequency B1 field distribution across the sampling volume, which can affect the quantification analysis. An effective and straightforward approach to metabolic profiling of soft tissue with spatial and chemical shift resolution.