Utilizing microalgal hydrolysate from dairy wastewater-grown Chlorella sorokiniana SU-1 as sustainable feedstock for polyhydroxybutyrate and 0-carotene production by engineered Rhodotorula glutinis #100-29

被引：5

作者：

Kusmayadi, Adi ^{[1
]}

Huang, Chi-Yu ^{[2
,3
]}

Leong, Yoong Kit ^{[1
,2
]}

Yen, Hong-Wei ^{[1
]}

Lee, Duu-Jong ^{[4
]}

Chang, Shu ^{[1
,2
,5
,6
]}

机构：

[1] Tunghai Univ, Dept Chem & Mat Engn, Taichung, Taiwan

[2] Tunghai Univ, Res Ctr Smart Sustainable Circular Econ, Taichung, Taiwan

[3] Tunghai Univ, Dept Environm Sci & Engn, Taichung, Taiwan

[4] City Univ Hong Kong, Dept Mech Engn, Kowloon Tang, Hong Kong, Peoples R China

[5] Natl Cheng Kung Univ, Dept Chem Engn, Tainan, Taiwan

[6] Yuan Ze Univ, Dept Chem Engn & Mat Sci, Chungli, Taiwan

来源：

BIORESOURCE TECHNOLOGY | 2023年 / 384卷

关键词：

Microalgae; Acidic hydrolysis; Detoxification; Yeast fermentation; Polyhydroxybutyrate; 0-carotene; LOOP AIRLIFT BIOREACTOR; DETOXIFICATION; OPTIMIZATION; BIOMASS; SACCHARIFICATION; BIOCONVERSION; ACCUMULATION; PRETREATMENT; BUTANOL; OILS;

D O I：

10.1016/j.biortech.2023.129277

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The objective of this study was to explore the potential of utilizing Chlorella sorokiniana SU-1 biomass grown on dairy wastewater-amended medium as sustainable feedstock for the biosynthesis of 0-carotene and polyhydroxybutyrate (PHB) by Rhodotorula glutinis #100-29. To break down the rigid cell wall, 100 g/L of microalgal biomass was treated with 3% sulfuric acid, followed by detoxification using 5% activated carbon to remove the hydroxymethylfurfural inhibitor. The detoxified microalgal hydrolysate (DMH) was used for flask-scale fermentation, which yielded a maximum biomass production of 9.22 g/L, with PHB and 0-carotene concentration of 897 mg/L and 93.62 mg/L, respectively. Upon scaling up to a 5-L fermenter, the biomass concentration increased to 11.2 g/L, while the PHB and 0-carotene concentrations rose to 1830 mg/L and 134.2 mg/L. These

引用

页数：8