Pb(II) bioremediation using fresh algal-bacterial aerobic granular sludge and its underlying mechanisms highlighting the role of extracellular polymeric substances

Lead (Pb) discharged from rural industries poses a significant threat to the environment and human health. Algal-bacterial aerobic granular sludge (A-B AGS) is a promising alternative for sewage treatment with high efficiency and good settleability. In this study, Pb(II) biosorption using fresh A-B AGS was investigated for the first time. The important role of extracellular polymeric substances (EPS) was revealed with the involved mechanisms being clarified. The desorbents for Pb recovery from Pb-loaded A-B AGS were also screened. Results showed that A-B AGS has an excellent maximum Pb adsorption capacity of 72.4 mg center dot g(-1) at pH 6.0. EPS plays an important role in keeping microbial activity, Pb bonding, and providing metal ions (Ca, Na and Mg) for Pb ion exchanges. Electrostatic interaction, ion exchange, and bonding to functional groups may occur orderly in the Pb biosorption process and the formation of pyromorphite (Pb-5(PO4)(3)Cl) contributes to Pb biosorption. About 66 % of the adsorbed Pb was accumulated in the A-B AGS microbial cells. Na(2)EDTA (0.05 M) can recover 60.3 % of the loaded Pb with the highest microbial activity of granules being remained. All the findings will provide the theoretical basis for the large-scale application of A-B AGS to bioremediate Pb(II)-containing wastewater.