Proof of Concept of a Novel Multiepitope Recombinant Protein for the Serodiagnosis of Patients with Chagas Disease

被引：4

作者：

Machado, Juliana Martins ^{[1
]}

Pereira, Isabela Amorim Goncalves ^{[2
]}

Maia, Ana Clara Gontijo ^{[1
]}

Francisco, Mariana Ferraz Chaves ^{[1
]}

Nogueira, Lais Moreira ^{[1
]}

Gandra, Isadora Braga ^{[1
]}

Ribeiro, Anna Julia ^{[1
]}

Silva, Kamila Alves ^{[1
]}

Resende, Carlos Ananias Aparecido ^{[1
]}

da Silva, Jonatas Oliveira ^{[1
]}

dos Santos, Michelli ^{[1
]}

Goncalves, Ana Alice Maia ^{[2
]}

Tavares, Grasiele de Sousa Vieira ^{[2
]}

Chavez-Fumagalli, Miguel Angel ^{[3
]}

Campos-da-Paz, Mariana ^{[4
]}

Giunchetti, Rodolfo Cordeiro ^{[5
]}

Rocha, Manoel Otavio da Costa ^{[2
]}

Chaves, Ana Thereza ^{[2
]}

Coelho, Eduardo Antonio Ferraz ^{[2
]}

Galdino, Alexsandro Sobreira ^{[1
]}

机构：

[1] Univ Fed Sao Joao Del Rei, Lab Biotecnol Microrganismos, BR-35501296 Divinopolis, MG, Brazil

[2] Univ Fed Minas Gerais, Fac Med, Programa Posgrad Ciencias Saude Infectol & Med Tr, BR-30130100 Belo Horizonte, MG, Brazil

[3] Univ Catolica Santa Maria, Vicerrectorado Invest, Computat Biol & Chem Res Grp, Arequipa 04000, Peru

[4] Univ Fed Sao Joao Del Rei, Lab NanoBiotecnol & Bioat, Sebastiao Goncalves Coelho 400, Divinopolis 355901296, MG, Brazil

[5] Univ Fed Minas Gerais, Dept Morfol, Lab Biol Interacoes Celulares, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil

来源：

PATHOGENS | 2023年 / 12卷 / 02期

关键词：

multiepitope; recombinant protein; immunodiagnosis; Chagas disease; TRYPANOSOMA-CRUZI INFECTION; IN-HOUSE ELISA; DIAGNOSIS; MANAGEMENT; ANTIGENS; PEPTIDE;

D O I：

10.3390/pathogens12020312

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Chagas disease remains a neglected disease that is considered to be a public health problem. The early diagnosis of cases is important to improve the prognosis of infected patients and prevent transmission. Serological tests are the method of choice for diagnosis. However, two serological tests are currently recommended to confirm positive cases. In this sense, more sensitive and specific serological tests need to be developed to overcome these current diagnosis problems. This study aimed to develop a new recombinant multiepitope protein for the diagnosis of Chagas disease, hereafter named rTC. The rTC was constructed based on amino acid sequences from different combinations of Trypanosoma cruzi antigens in the same polypeptide and tested using an enzyme-linked immunosorbent assay (ELISA) to detect different types of Chagas disease. rTC was able to discriminate between indeterminate (IND) and cardiac (CARD) cases and cross-reactive diseases, as well as healthy samples, with 98.28% sensitivity and 96.67% specificity, respectively. These data suggest that rTC has the potential to be tested in future studies against a larger serological panel for the diagnosis of Chagas disease.

引用

页数：11

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