Tahibacter soli sp. nov., isolated from soil and Tahibacter amnicola sp. nov., isolated from freshwater

Two Gram-stain- negative, facultative aerobic, catalase- and oxidase-positive, and non-motile rod bacteria, strains BLT and W38(T), that were isolated from soil and freshwater, respectively, were taxonomically characterized. Both strains optimally grew at 30 degrees C and pH 7.0 in Reasoner's 2A medium and contained ubiquinone-8 as the sole respiratory quinone. As major fatty acids (>10%), strain BLT contained iso-C-15:0 and summed features 3 and 9 (comprising iso-C-15:0 2-OH and/or C-16 :1 omega 7c/omega 6c and iso-C-17:1 omega 9c and/or C-16:0 10-methyl, respectively), whereas strain W38(T) contained iso-C-15:0, iso-C-16:0 and summed feature 9. Diphosphatidylglycerol and phosphatidylmonomethylethanolamine as major polar lipids and phosphatidylethanolamine and phosphatidylglycerol as minor polar lipids were detected in both strains. The DNA G+C contents of strains BLT and W38(T) were 68.3 and 65.3%, respectively. Phylogenetic analyses based on 16S rRNA gene and genome sequences revealed that strains BLT and W38(T) formed a tight phylogenetic lineage with Tahibacter species, and they shared 98.8% 16S rRNA gene sequence similarity and 75.5 % average nucleotide identity (ANI) and 16.6 % digital DNA-DNA hybridization (dDDH) values, indicating that they are different species. Strains BLT and W38(T) were most closely related to Tahibacter caeni BUT-6(T) and Tahibacter aquaticus PYM5-11(T) with 97.7 and 98.0 % 16S rRNA gene sequence similarities, respectively. ANI and dDDH values between strain BLT and T. caeni BUT-6(T) and between strain W38(T) and T. aquaticus DSM 21667(T) were 78.5 and 21.6% and 75.3 and 21.0%, respectively. Based on their phenotypic, chemotaxonomic and genomic properties, strains BLT and W38(T) represent two different novel species of the genus Tahibacter, for which the names Tahibacter soli sp. nov. and Tahibacter amnicola sp. nov. are proposed. The type strains of T. soli and T. amnicola are BLT (=KACC 22831(T)=JCM 35402(T)) and W38(T) (=KACC 22832(T)=JCM 35749(T)), respectively.