Effect of drought acclimation on antioxidant system and polyphenolic content of Foxtail Millet (Setaria italica L.)

The impact of climate change-induced drought stress on global food security and environmental sustainability is a serious concern. While previous research has highlighted the potential benefits of drought hardening in improving plants' ability to withstand drought, the exact underlying physiological mechanisms in millet plants (Setaria italica L.) have not been explored. This study aimed to investigate the impact of drought hardening on antioxidant defense and polyphenol accumulation in different millet genotypes ('PI 689680' and 'PI 662292') subjected to different treatments: control (unstressed), drought acclimation (two stress episodes with recovery), and non-acclimation (single stress episode with no recovery). The results showed that drought stress led to higher levels of polyphenols and oxidative damage, as indicated by increased phenolic, flavonoid, and anthocyanin levels. Non-acclimated (NA) plants experienced more severe oxidative damage and inhibition of enzymes associated with the ascorbate glutathione cycle compared to drought-acclimated plants. NA plants also exhibited a significant reduction in photosynthesis and tissue water content. The expression of genes related to antioxidants and polyphenol synthesis was more pronounced in non-acclimated plants. The study demonstrated that drought hardening not only prepared plants for subsequent drought stress but also mitigated damage caused by oxidative stress in plant physiology. Drought-acclimated (DA) plants displayed improved drought tolerance, as evidenced by better growth, photosynthesis, antioxidant defense, polyphenol accumulation, and gene expression related to antioxidants and polyphenol synthesis. In conclusion, the research advocates for the use of drought hardening as an effective strategy to alleviate the negative impacts of drought-induced metabolic disturbances in millet.