Efficacy of Phase I and Phase II Coxiella burnetii Bacterin Vaccines in a Pregnant Ewe Challenge Model

被引:6
|
作者
Williams-Macdonald, Sarah. E. E. [1 ,6 ]
Mitchell, Mairi [1 ,7 ]
Frew, David [1 ]
Palarea-Albaladejo, Javier [2 ,8 ]
Ewing, David [2 ]
Golde, William. T. T. [1 ]
Longbottom, David [1 ]
Nisbet, Alasdair. J. J. [1 ]
Livingstone, Morag [1 ]
Hamilton, Clare. M. M. [1 ]
Fitzgerald, Stephen. F. F. [1 ]
Buus, Soren [3 ]
Bach, Emil [3 ]
Dinkla, Annemieke [4 ]
Roest, Hendrik-Jan [4 ,9 ,10 ]
Koets, Ad. P. P. [4 ,5 ]
McNeilly, Tom. N. N. [1 ]
机构
[1] Moredun Res Inst, Pentlands Sci Pk, Bush Loan, Penicuik EH26 0PZ, Midlothian, Scotland
[2] JCMB, Biomath & Stat Scotland, Kings Bldg, Peter Guthrie Tait Rd, Edinburgh EH9 3FD, Scotland
[3] Univ Copenhagen, Fac Hlth & Med Sci, Dept Immunol & Microbiol, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark
[4] Wageningen Biovet Res, Host Pathogen Interact & Diagnost, Dept Bacteriol, Houtribweg 39, NL-8221 RA Lelystad, Netherlands
[5] Univ Utrecht, Fac Vet Med, Dept Populat Hlth Sci, Yalelaan 7, NL-3584 CL Utrecht, Netherlands
[6] Life Arc, Nine Edinburgh BioQuarter, 9 Little France Rd, Edinburgh EH16 4UX, Scotland
[7] Royal Infirm Edinburgh NHS Trust, STEC Reference Lab, Scottish E ColiO157, Edinburgh EH16 4SA, Scotland
[8] Univ Girona, Dept Comp Sci Appl Math & Stat, Girona 17003, Spain
[9] Minist Agr Nat & Food Qual, Bezuidenhoudseweg 73, NL-2594 AC The Hague, Netherlands
[10] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Yalelaan 1, NL-3584 CL Utrecht, Netherlands
基金
英国生物技术与生命科学研究理事会;
关键词
Q-fever; Coxiella burnetii; vaccine; sheep; phase I; phase II; Q-FEVER; CHLAMYDOPHILA-ABORTUS; SHEEP; INFECTION; GOATS; LIPOPOLYSACCHARIDE; VACCINATION; REDUCTION; VARIANTS; OUTBREAK;
D O I
10.3390/vaccines11030511
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The bacterium Coxiella burnetii can cause the disease Q-fever in a wide range of animal hosts. Ruminants, including sheep, are thought to play a pivotal role in the transmission of C. burnetii to humans; however, the only existing livestock vaccine, namely, Coxevac((R)) (Ceva Animal Health Ltd., Libourne, France), a killed bacterin vaccine based on phase I C. burnetii strain Nine-Mile, is only approved for use in goats and cattle. In this study, a pregnant ewe challenge model was used to determine the protective effects of Coxevac((R)) and an experimental bacterin vaccine based on phase II C. burnetii against C. burnetii challenge. Prior to mating, ewes (n = 20 per group) were vaccinated subcutaneously with either Coxevac((R)), the phase II vaccine, or were unvaccinated. A subset of pregnant ewes (n = 6) from each group was then challenged 151 days later (similar to 100 days of gestation) with 10(6) infectious mouse doses of C. burnetii, Nine-Mile strain RSA493. Both vaccines provided protection against C. burnetii challenge as measured by reductions in bacterial shedding in faeces, milk and vaginal mucus, and reduced abnormal pregnancies, compared to unvaccinated controls. This work highlights that the phase I vaccine Coxevac((R)) can protect ewes against C. burnetii infection. Furthermore, the phase II vaccine provided comparable levels of protection and may offer a safer and cost-effective alternative to the currently licensed vaccine.
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页数:19
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