Salidroside suppresses proliferation and migration in prostate cancer via the PI3K/AKT pathway

被引:3
|
作者
Liu, Ru-Han [1 ]
Ma, Teng-Fei [2 ,3 ]
Yang, Qin [3 ,4 ]
Xiao, Wen-Chang [3 ,4 ]
Yin, Lu [3 ]
Yin, Miao [3 ]
Zhang, Jin-Song [5 ]
Wang, Chi-Hua [6 ]
机构
[1] Huanggang Cent Hosp, Dept Combine Tradit Chinese & Western Med, Huanggang, Hubei, Peoples R China
[2] Huanggang Cent Hosp, Dept Neurol, Huanggang, Peoples R China
[3] Huanggang Inst Translat Med, Huanggang, Hubei, Peoples R China
[4] Huanggang Cent Hosp, Dept Cardiovasc Surg, Huanggang, Hubei, Peoples R China
[5] Huanggang Cent Hosp, Dept Urol, Huanggang, Peoples R China
[6] Huanggang Dis Control Ctr, Huanggang, Hubei, Peoples R China
关键词
Salidroside; prostate cancer; PC3; DU145; PI3K/AKT; IN-VITRO; CELLS; STATISTICS; APOPTOSIS; CHINA; MODEL;
D O I
10.3233/CBM-220454
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
BACKGROUND: Prostate cancer (PCa) is one of the most common malignancies in men. PCa is difficult to detect in its early stages, and most patients are diagnosed in the middle to late stages. At present, drug therapy for advanced PCa is still insufficient. Some patients develop drug resistance in the later stage of therapy, which leads to tumor recurrence, metastasis and even treatment failure. Therefore, it is crucial to find new and effective drugs to treat prostate cancer. OBJECTIVE: The aim of this study was to investigate the anti-cancer effect of salidroside, an active ingredient in a traditional Chinese herbal medicine, on PCa. METHODS: Two human PCa cell lines, PC3 and DU145, were cultured and treated with salidroside. Cell viability and proliferation ability were analyzed through CCK-8 and colony assays, and cell migration ability was detected by Transwell and Scratch assays. RT-PCR and WB were used to detected the expression levels of moleculars related to cell proliferation, apoptosis, migration, and AKT signaling pathway. Forthmore, we performed rescue experiments with agonist to verify the affected signaling pathway. RESULTS: Salidroside inhibited the proliferation, colony formation, and migration of PCa cells. Meanwhile, apoptosis of PCa cells was enhanced. Moreover, salidroside inhibited PI3K/AKT pathway in PCa cells. The treatment of AKT agonist 740Y-P abrogated the inhibitory effect of salidroside on the PI3K/AKT signaling pathway. CONCLUSIONS: Our study demonstrated that in PCa cells, salidroside inhibites proliferation and migration and promots apoptosis via inhibiting PI3K/AKT pathway.
引用
收藏
页码:321 / 332
页数:12
相关论文
共 50 条
  • [1] Fetuin B overexpression suppresses proliferation, migration, and invasion in prostate cancer by inhibiting the PI3K/AKT signaling pathway
    Zhan, Kai
    Liu, Rui
    Tong, Hang
    Gao, Shun
    Yang, Guang
    Hossain, Arman
    Li, Tinghao
    He, Weiyang
    [J]. BIOMEDICINE & PHARMACOTHERAPY, 2020, 131
  • [2] LncRNA NKILA suppresses colon cancer cell proliferation and migration by inactivating PI3K/Akt pathway
    Huang, Jian
    Zhao, Lingfeng
    Chen, Wei
    Duan, Jian
    Shrestha, Dibesh
    Zhou, Ruize
    Tuo, Xiaoyu
    Zhu, Zhu
    [J]. TRANSLATIONAL CANCER RESEARCH, 2018, 7 (06) : 1431 - 1438
  • [3] Apatinib suppresses the proliferation and apoptosis of gastric cancer cells via the PI3K/Akt signaling pathway
    Jia, Xiaoqiong
    Wen, Zhenping
    Sun, Qiuying
    Zhao, Xiaohua
    Yang, Hao
    Shi, Xiaoyu
    Xin, Tao
    [J]. JOURNAL OF BUON, 2019, 24 (05): : 1985 - 1991
  • [4] TIPE2 Overexpression Suppresses the Proliferation; Migration, and Invasion in Prostate Cancer Cells by Inhibiting PI3K/Akt Signaling Pathway
    Lu, Qiang
    Liu, Zhe
    Li, Zhuo
    Chen, Jia
    Liao, Zhi
    Wu, Wan-rui
    Li, Yuan-wei
    [J]. ONCOLOGY RESEARCH, 2016, 24 (05) : 305 - 313
  • [5] Pentamidine inhibits proliferation, migration and invasion in endometrial cancer via the PI3K/AKT signaling pathway
    Lin, Lin
    Gao, Yunan
    Hu, Xiaochen
    Ouyang, Jiabao
    Liu, Chunbo
    [J]. BMC WOMENS HEALTH, 2022, 22 (01)
  • [6] Pentamidine inhibits proliferation, migration and invasion in endometrial cancer via the PI3K/AKT signaling pathway
    Lin Lin
    Yunan Gao
    Xiaochen Hu
    Jiabao Ouyang
    Chunbo Liu
    [J]. BMC Women's Health, 22
  • [7] The PI3K/AKT pathway in the pathogenesis of prostate cancer
    Chen, Huixing
    Zhou, Lan
    Wu, Xiaorong
    Li, Rongbing
    Wen, Jiling
    Sha, Jianjun
    Wen, Xiaofei
    [J]. FRONTIERS IN BIOSCIENCE-LANDMARK, 2016, 21 : 1084 - 1091
  • [8] Osthole induces apoptosis and suppresses proliferation via the PI3K/Akt pathway in intrahepatic cholangiocarcinoma
    Zhu, Xingyang
    Song, Xiaoling
    Xie, Kun
    Zhang, Xue
    He, Wei
    Liu, Fubao
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2017, 40 (04) : 1143 - 1151
  • [9] Eriodictyol Suppresses Gastric Cancer Cells via Inhibition of PI3K/AKT Pathway
    Shan, Hui
    Zhang, Xin
    Mi, Yalu
    Jia, Jihui
    Wang, Bo
    Yang, Qing
    [J]. PHARMACEUTICALS, 2022, 15 (12)
  • [10] Ligustilide Inhibits the PI3K/AKT Signalling Pathway and Suppresses Cholangiocarcinoma Cell Proliferation, Migration, and Invasion
    Wu, Yue
    Rong, Li
    Zhang, Suifeng
    He, Yuxi
    Song, Na
    Zuo, Guoqing
    Mei, Zhechuan
    [J]. RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY, 2024,