Comparative Diagnostic Performance of Microscopy, SD-Bioline Rapid Diagnostic Test, and Polymerase Chain Reaction in the Detection of Malaria Infection among Pregnant Women at Delivery in Kumba Health District Area in the Southwest Region of Cameroon

被引:0
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作者
Ngemani Obase, Bekindaka [1 ,2 ]
Livo Forgu, Esemu [2 ,3 ,4 ]
Honore Awanakam, Awanakam [2 ,4 ,5 ]
Francis, Zeukeng [2 ,6 ]
Balonga Annie, Agnenga [2 ,5 ]
Loic, Nchankou [2 ,5 ]
Daiga Bigoga, Jude [2 ,5 ]
Leke, Rose [2 ]
Nsagha, Dickson Shey [7 ]
机构
[1] Univ Ibadan, Pan African Univ, Life & Earth Sci Inst Including Hlth & Agr, Ibadan, Nigeria
[2] Univ Yaounde I, Immunol & Mol Biol Lab, Biotechnol Ctr, Yaounde, Cameroon
[3] Univ Buea, Fac Hlth Sci, Dept Biomed Sci, Buea, Cameroon
[4] Inst Med Res & Med Plant Studies, Ctr Res Emerging & Reemerging Dis, Yaounde, Cameroon
[5] Univ Yaounde I, Fac Sci, Dept Biochem, Yaounde, Cameroon
[6] Univ Buea, Fac Sci, Dept Biochem & Biotechnol, Buea, Cameroon
[7] Univ Buea, Fac Hlth Sci, Dept Publ Hlth & Hyg, Buea, Cameroon
关键词
INCREASED PREVALENCE; HEMOGLOBIN LEVELS; HIV; IMPACT;
D O I
10.1155/2023/2056524
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Introduction. Malaria during pregnancy is a major cause of morbidity and mortality in sub-Saharan Africa. Microcopy and rapid diagnostic test (RDT) recommended by the World Health Organization for clinical diagnosis have poor sensitivity to detect individuals with very low levels of parasitemia. Previous studies have shown that malaria in pregnancy is associated with mastitis and excessive uterine blood loss during delivery. However, information evaluating the performance of these tools in detecting malaria in pregnancy at the national level is limited. This study therefore evaluates the performance of microscopy, RDT, and nested polymerase chain reaction (nPCR) in the detection of pregnancy-associated malaria at delivery. Methods. A total of 227 participants constituting of 201 pregnant women without and 26 with HIV were recruited from five health facilities within the Kumba health district area. Mother venous and cord blood were collected at delivery to test for malaria using the thick-film microscopy, SD-bioline RDT, and 18SrRNA-nested PCR. Results. The percentage of malaria-positive cases detected by thick-film microcopy (TFM), RDT, and PCR in pregnant women with and without HIV was 7.69%, 53.85%, and 50% and 3.48%, 23.38%, and 49.25%, respectively. Plasmodium falciparum was detected in 1.99% cord blood samples of women without HIV by PCR. The positivity rate in at least two of the test methods (composite positive) was 42.31% for women with and 19.90% for women without HIV. The sensitivity of TFM and RDT when using PCR as a reference was 7.21% and 49.00%, respectively, in all samples. The specificity was 99.14% and 90.55% with kappa values of 0.064 and 0.461, respectively. When using the composite test as a reference, the sensitivity of TFM, RDT, and nPCR was 15.69%, 94.12%, and 100%, respectively. Specificity was 99.43%, 93.18%, and 65.34% with kappa values of 0.213, 0.821, and 0.458, respectively. Conclusion. This study shows that PCR is more sensitive in the detection of malaria parasite followed by SD-bioline RDT kit. However, in resource-limited settings where access to molecular diagnosis of malaria is a problem, RDT should be considered as the first option to microscopy in the diagnosis of malaria.
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页数:17
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  • [1] SD-Bioline malaria rapid diagnostic test performance and time to become negative after treatment of malaria infection in Southwest Nigerian Children
    Orimadegun, Adebola Emmanuel
    Dada-Adegbola, Hannah O.
    Michael, Obaro S.
    Adepoju, Akinlolu Adedayo
    Funwei, Roland Ibenipere
    Olusola, Fiyinfoluwa Ibukun
    Ajayi, IkeOluwapo O.
    Ogunkunle, Oluwatoyin O.
    Ademowo, Olusegun George
    Jegede, Ayodele Samuel
    Baba, Ebenezer
    Hamade, Prudence
    Webster, Jayne
    Chandramohan, Daniel
    Falade, Catherine Olufunke
    [J]. ANNALS OF AFRICAN MEDICINE, 2023, 22 (04) : 470 - 480
  • [2] Performance of rapid diagnostic test, light microscopy, and polymerase chain reaction in pregnant women with asymptomatic malaria in Nigeria
    Adebusuyi, Sunday A.
    Olorunfemi, Adedolapo Blessing
    Fagbemi, Kaossarath Adedjoke
    Nderu, David
    Amoo, Abimbola O. J.
    Thomas, Bolaji N.
    Velavan, Thirumalaisamy P.
    Ojurongbe, Olusola
    [J]. IJID REGIONS, 2024, 12
  • [3] EVALUATION OF THE PERFORMANCE OF SD-BIOLINE MALARIA RAPID DIAGNOSTIC TEST CARRIED OUT BY COMMUNITY MEDICINE DISTRIBUTORS AGAINST MICROSCOPY AND PCR IN THE DIAGNOSIS OF MALARIA IN SOUTHWEST NIGERIA
    Falade, Catherine Olufunke
    Ajayi, IkeOluwapo Oyeneye
    Jegede, Ayodele Samuel
    Afonne, Chinenye
    Ogunsesin, Tolulope
    Funwei, Roland I. Ibenipere
    Ojurongbe, Olusola
    Singlovic, Jan
    Gomes, Melba
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2019, 101 : 277 - 277
  • [4] Malaria Diagnostics: A Comparative Study of Blood Microscopy, a Rapid Diagnostic Test and Polymerase Chain Reaction in the Diagnosis of Malaria
    Runsewe-Abiodun, Iyabo T.
    Efunsile, Michael
    Ghebremedhin, Beniam
    Sotimehin, Adetoun S.
    Ajewole, Jerome
    Akinleye, James
    Koenig, Brigite
    Koenig, Wolfang
    [J]. JOURNAL OF TROPICAL PEDIATRICS, 2012, 58 (02) : 163 - 164
  • [5] Performance comparison of two malaria rapid diagnostic test with real time polymerase chain reaction and gold standard of microscopy detection method
    Wardhani, Puspa
    Butarbutar, Trieva Verawaty
    Adiatmaja, Christophorus Oetama
    Betaubun, Amarensi Milka
    Hamidah, Nur
    Aryati
    [J]. INFECTIOUS DISEASE REPORTS, 2020, 12 (01) : 56 - 60
  • [6] Comparison of polymerase chain reaction, microscopy, and rapid diagnostic test in malaria detection in a high burden state (Odisha) of India
    Ahmad, Amreen
    Soni, Prahalad
    Kumar, Lalit
    Singh, Mrignendra Pal
    Verma, Anil Kumar
    Sharma, Anjana
    Das, Aparup
    Bharti, Praveen Kumar
    [J]. PATHOGENS AND GLOBAL HEALTH, 2021, 115 (04) : 267 - 272
  • [7] Comparison of Rapid Diagnostic Test, Microscopy, and Polymerase Chain Reaction for the Detection of Plasmodium falciparum Malaria in a Low-Transmission Area, Jazan Region, Southwestern Saudi Arabia
    Madkhali, Aymen M.
    Ghzwani, Ahmad Hassn
    Al-Mekhlafi, Hesham M.
    [J]. DIAGNOSTICS, 2022, 12 (06)
  • [8] Diagnostic Performance of Malaria Rapid Diagnostic Test and Microscopy Compared with PCR for Detection of Plasmodium falciparum Infections among Primary Schoolchildren in Kibiti District, Eastern Tanzania: An Area with Moderate Malaria Transmission
    Ngasala, Billy
    Mutemi, Doreen D.
    Mwaiswelo, Richard O.
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2019, 101 (04): : 809 - 811
  • [9] Detection of malaria infection in blood transfusion: a comparative study among real-time PCR, rapid diagnostic test and microscopy
    Hassanpour, Gholamreza
    Mohebali, Mehdi
    Raeisi, Ahmad
    Abolghasemi, Hassan
    Zeraati, Hojjat
    Alipour, Mohsen
    Azizi, Ebrahim
    Keshavarz, Hossein
    [J]. PARASITOLOGY RESEARCH, 2011, 108 (06) : 1519 - 1523
  • [10] Performance of rapid diagnostic test, blood-film microscopy and PCR for the diagnosis of malaria infection among febrile children from Korogwe District, Tanzania
    Coline Mahende
    Billy Ngasala
    John Lusingu
    Tai-Soon Yong
    Paminus Lushino
    Martha Lemnge
    Bruno Mmbando
    Zul Premji
    [J]. Malaria Journal, 15