Phenotypic and Molecular Characterization of a Hospital Outbreak Clonal Lineage of Salmonella enterica Subspecies enterica serovar Mikawasima Containing blaTEM-1B and blaSHV-2 That Emerged on a Neonatal Ward, During the COVID-19 Pandemic

被引：0

作者：

Novak, Anita ^{[1
,2
,3
]}

Dzelalija, Mia ^{[4
]}

Goic-Barisic, Ivana ^{[1
,2
]}

Kovacic, Ana ^{[5
]}

Pirija, Mario ^{[1
]}

Maravic, Ana ^{[4
]}

Radic, Marina ^{[1
,2
]}

Marinovic, Jelena ^{[1
,2
]}

Rubic, Zana ^{[1
,2
]}

Carev, Merica ^{[2
,3
,5
,6
]}

Tonkic, Marija ^{[1
,2
]}

机构：

[1] Univ Hosp Split, Dept Clin Microbiol, Split, Croatia

[2] Univ Split, Sch Med, Split, Croatia

[3] EFWISG, ESCMID Food & Water Borne Infect Study Grp, CH-4010 Basel, Switzerland

[4] Univ Split, Fac Sci, Dept Biol, Split, Croatia

[5] Teaching Publ Hlth Inst Split Dalmatia Cty, Split, Croatia

[6] Univ Split, Dept Hlth Studies, Split, Croatia

来源：

MICROBIAL DRUG RESISTANCE | 2024年 / 30卷 / 03期

关键词：

Salmonella Mikawasima; whole-genome sequencing; MLST; bla(TEM-1B; bla(SHV-2; tet(D); FIELD GEL-ELECTROPHORESIS; ANTIMICROBIAL RESISTANCE;

D O I：

10.1089/mdr.2023.0132

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Nontyphoid salmonella can cause severe infections in newborns and is therefore declared a pathogen of major health significance at this age. The aim of the study was molecular and antimicrobial characterization of beta-lactamase-producing Salmonella Mikawasima outbreak clone on a Neonatal ward, University Hospital of Split (UHS), Croatia during the COVID-19 pandemic. From April 2020, until April 2023, 75 nonrepetitive strains of Salmonella Mikawasima were isolated from stool specimens and tested for antimicrobial resistance. All 75 isolates were resistant to ampicillin and gentamicin, while 98% of isolates were resistant to amoxicillin/clavulanic acid. A high level of resistance was observed to third-generation cephalosporins (36% to ceftriaxone and 47% to ceftazidime). Extended-spectrum beta-lactamase production was phenotypically detected by double-disk synergy test in 40% of isolates. Moderate resistance to quinolones was detected; 7% of isolates were resistant to pefloxacin and ciprofloxacin. All isolates were susceptible to carbapenems, chloramphenicol, and co-trimoxazole. Fourteen representative isolates, from 2020, 2021, 2022, and 2023, were analyzed with PFGE and all of them belong to the same clone. Whole-genome sequencing (WGS) analysis of three outbreak-related strains (SM1 and SM2 from 2020 and SM3 from 2023) confirmed that these strains share the same serotype (Mikawasima), multilocus sequence typing profile (ST2030), resistance genes [bla(TEM-1B), aac(6')-Iaa, aac(6')-Im, and aph(2'')-Ib)] and carry incompatibility group C (IncC) plasmid. Furthermore, the gene bla(SHV-2) was detected in SM1 and SM2. In summary, WGS analysis of three representative strains clearly demonstrates the persistence of beta-lactamase-producing Salmonella Mikawasima in UHS during the 4-year period.

引用

页码：118 / 126

页数：9