The road to a polio-free Uganda; contribution of the Expanded Program on Immunization Laboratory (EPI-LAB) at Uganda Virus Research Institute

被引：1

作者：

Nanteza, Mary B. ^{[1
]}

Tushabe, Phionah ^{[1
]}

Bukenya, Henry ^{[1
]}

Namuwulya, Prossy ^{[1
]}

Kabaliisa, Theopista ^{[1
]}

Birungi, Molly ^{[1
]}

Tibanagwa, Mayi ^{[1
]}

Ampeire, Immaculate ^{[2
]}

Kakooza, Proscovia ^{[1
]}

Katushabe, Edson ^{[3
]}

Bwogi, Josephine ^{[1
]}

Bakamutumaho, Barnabas ^{[1
]}

Nanyunja, Miriam ^{[4
]}

Byabamazima, Charles R. ^{[5
]}

机构：

[1] Uganda Virus Res Inst, Plot 51-59 Nakiwogo Rd,POB 49, Entebbe, Uganda

[2] Govt Uganda, Minist Hlth, Plot 6,Lourdel Rd,POB 7272, Kampala, Uganda

[3] World Hlth Org Off, Plot 60 Prince Charles Ave, Kampala, Uganda

[4] World Hlth Org AFRO, East & Southern Afr ESA, Nairobi 45335, Kenya

[5] World Hlth Org AFRO, East & Southern Afr ESA, 82-86 Enterprise Rd,POB BE 773, Belvedere, Harare, Zimbabwe

来源：

AFRICAN HEALTH SCIENCES | 2023年 / 23卷 / 03期

关键词：

Poliovirus; eradication; acute flaccid paralysis; laboratory surveillance; Uganda; ACUTE FLACCID PARALYSIS; POLIOVIRUSES; ERADICATION; CULTURE;

D O I：

10.4314/ahs.v23i3.23

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: The control of poliomyelitis in Uganda dates back as far as 1950 and acute flaccid paralysis (AFP) surveillance has since been used as a criterion for identifying wild polioviruses. Poliovirus isolation was initially pursued through collaborative research however, in 1993, the Expanded Program on Immunization Laboratory (EPI-LAB) was established as a member of the Global Poliovirus Laboratory Network (GPLN) and spearheaded this activity at Uganda Virus Research Institute.Objectives: The aim of this report is to document the progress and impact of the EPI-LAB on poliovirus eradication in Uganda.Methods: Poliovirus detection and identification were achieved fundamentally through tissue culture and intra-typic differentia- tion of the poliovirus based on the real-time reverse transcriptase polymerase chain reaction (rRT PCR). The data obtained was entered into the national AFP database and analysed using EpiInfoTM statistical software.Results: Quantitative and qualitative detection of wild and Sabin polioviruses corresponded with the polio campaigns. The WHO target indicators for AFP surveillance were achieved essentially throughout the study period.Conclusion: Virological tracking coupled with attaining standard AFP surveillance indicators has been pivotal in achieving and maintaining the national wild polio-free status. Laboratory surveillance remains key in informing the certification process of polio eradication.

引用

页码：186 / 196

页数：11