Tin Chloride Mediated Grinding Method for Preparation of 2,4-Disubstituted Benzodiazepine Scaffolds Their In Silico, and In Vitro Cell Viability Studies

The easy grinding approach using Tin chloride-mediated aryl, heteryl substituted benzodiazepine series production was the main focus of this study. We employed silica gel and dried Luffa cylindrica for the first time to better separate the named components during the purification of the crude product. Here, the heterocyclic ring serves as an anchoring group, the aromatic ring serves as a hydrophobic group, and the diazepine group serves as a linker. Prediction algorithms indicate that these compounds have antineoplastic action and operate as CYP2A11 substrates. The IC50 values of benzodiazepines (X): 1.886, (XI): 1.743, and (XII): 1.7325 against mouse breast cancer cell line (4T1) are better than control (untreated cells) and reference Lipofectamine 2000 (L2K).