Development of novel fluorescent probes to detect and quantify specific reactive oxygen species

Hydrogen peroxide (H2O2) plays important roles in redox signaling and oxidative stress, and its dynamic concentration is critical to human health and diseases. Here we report the synthesis of a novel fluorescent probe based on pyridine-coumarin core 3-(2-pyridyl)-7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)c hromen-2-one (P182) for quantitative measurement of H2O2. This fluorometric probe displays a fluores-cence turn-on response in the process of deprotection of arylboronate to phenol in the presence of H2O2. It could offer good performances in terms of sensitivity and response time. Moreover, cytotoxicity studies on various model cell lines proved the non-toxic activity of the tested sensor allowing its use in vivo stud-ies. This study provides research on molecular fluorescence probe for the detection of H2O2.(c) 2022 Elsevier B.V. All rights reserved.