Quality of the sample-based RNA determines the real-time RT-PCR results in the laboratory diagnosis of COVID-19

被引：0
|
作者：
Shihab, Sibra R. M. [1 ,2 ]
Iqbal, Bushran N. [1 ]
Arunasalam, Shiyamalee [1 ]
Noordeen, Faseeha [1 ]
机构：
[1] Univ Peradeniya, Fac Med, Dept Microbiol, Diag & Res Virol Lab, Peradeniya, Sri Lanka
[2] Univ Peradeniya, Postgrad Inst Sci, Peradeniya, Sri Lanka
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2024年 / 108卷 / 01期
关键词：
Inconclusive results; Ct value; SARS-CoV-2; COVID-19; RNA concentration;
D O I：
10.1016/j.diagmicrobio.2023.116076
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Effect of the quality of sample-based RNA on COVID-19 real-time RT-PCR results was investigated. The purity of the extracts was dependent on the extraction method (P<0.0001) and affected the test interpretations (P = 0.002). Gross RNA concentration negatively correlated with Ct values (P < 0.0001). The presence of impurities contributed to inconclusive test results.
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页数：3
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