Storage of Extended Boar Semen at 5 °C Inhibits Growth of Multi-Drug Resistant Serratia marcescens and Klebsiella oxytoca while Maintaining High Sperm Quality

被引：7

作者：

Maassen, Isabel Katharina ^{[1
]}

Luther, Anne-Marie ^{[1
]}

Verspohl, Jutta ^{[2
]}

Waberski, Dagmar ^{[1
]}

机构：

[1] Univ Vet Med Hannover, Clin Pigs & Small Ruminants, Unit Reprod Med, Bunteweg 15, D-30559 Hannover, Germany

[2] Univ Vet Med Hannover, Inst Microbiol, Bischofsholer Damm 15, D-30173 Hannover, Germany

来源：

ANTIBIOTICS-BASEL | 2023年 / 12卷 / 05期

关键词：

Serratia marcescens; Klebsiella oxytoca; boar semen; antibiotic resistance; semen preservation; ESCHERICHIA-COLI; ADHERENCE;

D O I：

10.3390/antibiotics12050857

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Multi-drug antibiotic resistance of Serratia (S.) marcescens and Klebsiella (K.) oxytoca in boar semen is an emerging threat to pig reproduction and the environment. The aim of this study is to examine the efficiency of a novel hypothermic preservation method to inhibit the growth of these bacterial species in extended boar semen and to maintain the sperm quality. The semen samples extended in an antibiotic-free Androstar Premium extender were spiked with similar to 10(2) CFU/mL of S. marcescens or K.oxytoca. Storage at 5 degrees C for 144 h inhibited the growth of both bacterial species and maintained the sperm quality, whereas bacterial counts increased to more than 10(10) CFU/mL in the 17 degrees C samples used as positive controls. This was accompanied by an increase in the sperm agglutination and the loss of motility and membrane integrity. We conclude that hypothermic storage is a promising tool to combat resistant bacteria in boar semen and to contribute to the One Health approach.

引用

页数：7