Co-cultivation of Chaetoceros calcitrans and Arthrospira platensis growing on palm oil mill effluent under outdoor condition to produce fucoxanthin and c-phycocyanin

Fucoxanthin from microalgae is promising since the productivity is higher compared to macroal-gae. However, dynamic environmental and nutritional factors in large scale and outdoor condi-tion still limiting the production. This research investigated co-cultivation of Chaetoceros calci-trans and Arthrospira platensis growing under outdoor condition and evaluated the pigment pro-duction when using palm oil mill effluent (POME) as medium growth. Results showed that co -cultivation strategy produced abundant biomass when growing up to 30 PSU salinity. The addi-tion of POME to cultivation medium reduced fucoxanthin production, while C-phycocyanin (C-PC) production could be maintained. Mixed culture produced 27 mg/L C-PC and 5.4 mg/L fucox-anthin by using synthetic medium. The optimal condition for co-cultivation was 33% POME, 100 mg/L urea, and 17 PSU salinity to produce 1.7 mg/L fucoxanthin and 25 mg/L C-PC.