Characteristics of copper oxide and tin oxide nanoparticles produced by using pulsed laser ablation method and their application as an antibacterial agent

The synthesis of copper oxide (CuO) and tin oxide (SnO2) nanoparticles was successfully carried out using the neodymium yttrium aluminum garnet (Nd:YAG) laser ablation method. In this study, a laser beam was focused on the surface of high-purity (99.9%) Cu and Sn plates placed into deionized water (DIW) and 0.2% carboxymethyl cellulose (CMC) liquid media, respectively, to produce colloidal CuO and SnO2 nanoparticles. CuO and SnO2 nanoparticle colloids were successfully produced with greenish and brownish colors, respectively. The colloidal nanoparticles produced in CMC media are more stable. Thus, the antibacterial test on E. coli bacteria uses CuO and SnO2 samples in CMC media. Antibacterial assessments were carried out using disc diffusion and liquid dilution methods equipped with total plate counting (TPC) techniques, with a sample variation of 26 ppm CuO, 78 ppm SnO2, and a CuO-SnO2 mixture of 5:56 ppm and 19:19 ppm samples. The results of the disc diffusion test yielded the diameter of the bacterial inhibition zone. The diameter of the inhibition zone in CuO 26 ppm, SnO2 78 ppm, and CuO-SnO2 5:56 ppm and 19:19 ppm samples, respectively, were 7.43 mm, 6.39 mm, 7.18 mm, and 6.54 mm, while the liquid dilution test equipped with TPC produced the percentage of bacterial degradation, respectively, of 98.93%, 97.60%, 98.47%, and 97.73%. The results show that all variations had the effectiveness of inhibiting and killing bacteria. From the results of various bacterial tests, colloidal CuO nanoparticles are more effective than the SnO2 nanoparticles.