Comprehensive analysis of the complete mitochondrial genomes of three Coptis species (C. chinensis, C. deltoidea and C. omeiensis): the important medicinal plants in China

被引:6
|
作者
Zhong, Furong [1 ,2 ]
Ke, Wenjia [1 ,2 ]
Li, Yirou [1 ,2 ]
Chen, Xiaoyan [1 ,2 ]
Zhou, Tao [1 ,2 ]
Xu, Binjie [3 ]
Qi, Luming [4 ,5 ]
Yan, Zhuyun [1 ,2 ]
Ma, Yuntong [1 ,2 ]
机构
[1] Chengdu Univ Tradit Chinese Med, State Key Lab Characterist Chinese Med Resources S, Chengdu, Peoples R China
[2] Chengdu Univ Tradit Chinese Med, Sch Pharm, Chengdu, Peoples R China
[3] Chengdu Univ Tradit Chinese Med, Innovat Inst Chinese Med & Pharm, Chengdu, Sichuan, Peoples R China
[4] Chengdu Univ Tradit Chinese Med, Sch Hlth Preservat & Rehabil, Chengdu, Sichuan, Peoples R China
[5] State Adm Tradit Chinese Med, Key Lab Tradit Chinese Med Regimen & Hlth, Chengdu, Sichuan, Peoples R China
来源
关键词
Coptis species; mitochondrial genome; repeat sequence; genome size variation; thermal acclimation; OXIDATIVE STRESS; HEAT-STRESS; EVOLUTION; RESPIRATION; MITOGENOMES; METABOLISM; MECHANISMS; SELECTION; ALIGNMENT; DYNAMICS;
D O I
10.3389/fpls.2023.1166420
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Coptis plants (Ranunculaceae) contain high levels of isoquinoline alkaloids and have a long history of medicinal use. Coptis species are of great value in pharmaceutical industries and scientific research. Mitochondria are considered as one of the central units for receiving stress signals and arranging immediate responses. Comprehensive characterizations of plant mitogenomes are imperative for revealing the relationship between mitochondria, elucidating biological functions of mitochondria and understanding the environmental adaptation mechanisms of plants. Here, the mitochondrial genomes of C. chinensis, C. deltoidea and C. omeiensis were assembled through the Nanopore and Illumina sequencing platform for the first time. The genome organization, gene number, RNA editing sites, repeat sequences, gene migration from chloroplast to mitochondria were compared. The mitogenomes of C. chinensis, C. deltoidea and C. omeiensis have six, two, two circular-mapping molecules with the total length of 1,425,403 bp, 1,520,338 bp and 1,152,812 bp, respectively. The complete mitogenomes harbors 68-86 predicted functional genes including 39-51 PCGs, 26-35 tRNAs and 2-5 rRNAs. C. deltoidea mitogenome host the most abundant repeat sequences, while C. chinensis mitogenome has the largest number of transferred fragments from its chloroplasts. The large repeat sequences and foreign sequences in the mitochondrial genomes of Coptis species were related to substantial rearrangements, changes in relative position of genes and multiple copy genes. Further comparative analysis illustrated that the PCGs under selected pressure in mitochondrial genomes of the three Coptis species mainly belong to the mitochondrial complex I (NADH dehydrogenase). Heat stress adversely affected the mitochondrial complex I and V, antioxidant enzyme system, ROS accumulation and ATP production of the three Coptis species. The activation of antioxidant enzymes, increase of T-AOC and maintenance of low ROS accumulation in C. chinensis under heat stress were suggested as the factors for its thermal acclimation and normal growth at lower altitudes. This study provides comprehensive information on the Coptis mitogenomes and is of great importance to elucidate the mitochondrial functions, understand the different thermal acclimation mechanisms of Coptis plants, and breed heat-tolerant varieties.
引用
收藏
页数:16
相关论文
共 50 条
  • [1] Characterization of the complete chloroplast genomes of three Chirita species (C. brachytricha, C. eburnea & C. liboensis) endemic to China
    Na Hou
    Gang Wang
    Cong-Rui Li
    Yang Luo
    Conservation Genetics Resources, 2018, 10 : 597 - 600
  • [2] The complete chloroplast genomes of three rare and endangered camellias (Camellia huana, C. liberofilamenta and C. luteoflora) endemic to Southwest China
    Gang Wang
    Yang Luo
    Na Hou
    Lun-Xiu Deng
    Conservation Genetics Resources, 2017, 9 : 583 - 585
  • [3] Three new species of Candida from apple cider: C. anglica, C. cidri and C. pomicola
    C.P. Kurtzman
    C.J. Robnett
    D. Yarrow
    Antonie van Leeuwenhoek, 2001, 80 : 237 - 244
  • [4] Cytological analyses of the karyotypes and chromosomes of three Colletotrichum species, C. orbiculare, C. graminicola and C. higginsianum
    Taga, Masatoki
    Tanaka, Kaoru
    Kato, Seiji
    Kubo, Yasuyuki
    FUNGAL GENETICS AND BIOLOGY, 2015, 82 : 238 - 250
  • [5] Dormancy in three Calanus species (C. finmarchicus, C. glacialis and C. hyperboreus) from the North Atlantic
    Hirche, HJ
    ADVANCES IN LIMNOLOGY, VOL 52: EVOLUTIONARY AND ECOLOGICAL ASPECTS OF CRUSTACEAN DIAPAUSE, 1998, 52 : 359 - 369
  • [6] NESTING BEHAVIOR OF THREE SPECIES OF CHLOROSPINGUS (C. FLAVIGULARIS, C. FLAVOPECTUS, AND C. PARVIROSTRIS) IN SOUTHEASTERN PERU
    Sanchez-Martinez, Manuel A.
    Londono, Gustavo A.
    WILSON JOURNAL OF ORNITHOLOGY, 2016, 128 (04): : 784 - 793
  • [7] Complete chloroplast genomes of Cerastium alpinum, C. arcticum and C. nigrescens: genome structures, comparative and phylogenetic analysis
    Milarska, Sylwia E.
    Androsiuk, Piotr
    Paukszto, Lukasz
    Jastrzebski, Jan P.
    Mazdziarz, Mateusz
    Larson, Keith W.
    Gielwanowska, Irena
    SCIENTIFIC REPORTS, 2023, 13 (01)
  • [8] The complete mitochondrial genomes of Cynomys leucurus and C. ludovicianus (Rodentia: Sciuridae)
    Li, Bingfei
    Yu, Danna
    Cheng, Hongyi
    Storey, Kenneth B.
    Zhang, Jiayong
    MITOCHONDRIAL DNA PART A, 2016, 27 (05) : 3295 - 3296
  • [9] Complete chloroplast genomes of Cerastium alpinum, C. arcticum and C. nigrescens: genome structures, comparative and phylogenetic analysis
    Sylwia E. Milarska
    Piotr Androsiuk
    Łukasz Paukszto
    Jan P. Jastrzębski
    Mateusz Maździarz
    Keith W. Larson
    Irena Giełwanowska
    Scientific Reports, 13
  • [10] Leaf Spot Caused by Colletotrichum siamense, C. fructicola, and C. aeschynomenes on Ixora chinensis in Guangxi, China
    Qin, Ruyun
    Zhang, Yanmiao
    Li, Qili
    Huang, Suiping
    Chen, Xiaolin
    Guo, Tangxun
    Tang, Lihua
    PLANT DISEASE, 2024, 108 (01)