Facile pretreatment strategies to biotransform Kans grass into nanocatalyst, cellulolytic enzymes, and fermentable sugars towards sustainable biorefinery applications

被引：3

作者：

Singh, Preeti ^{[1
]}

Srivastava, Neha ^{[2
]}

Mohammad, Akbar ^{[3
]}

Lal, Basant ^{[4
]}

Singh, Rajeev ^{[5
]}

Syed, Asad ^{[6
]}

Elgorban, Abdallah M. ^{[6
]}

Verma, Meenakshi ^{[7
]}

Mishra, P. K. ^{[2
]}

Gupta, Vijai Kumar ^{[8
,9
]}

机构：

[1] Indian Inst Technol BHU Varanasi, Technol Business Incubator, Varanasi 221005, Uttar Pradesh, India

[2] Indian Inst Technol BHU Varanasi, Dept Chem Engn & Technol, Varanasi 221005, Uttar Pradesh, India

[3] Yeungnam Univ, Sch Chem Engn, Gyongsan 38541, South Korea

[4] GLA Univ, Inst Appl Sci & Humanities, Dept Chem, Mathura 281406, Uttar Pradesh, India

[5] A Cent Univ, Dept Environm Sci, Jamia Millia Islamia, New Delhi 110025, India

[6] King Saud Univ, Coll Sci, Dept Bot & Microbiol, PO 2455, Riyadh 11451, Saudi Arabia

[7] Univ Ctr Res & Dev, Chandigarh Univ, Dept Chem, Mohali 140413, India

[8] Biorefining & Adv Mat Res Ctr, SRUC, Barony Campus, Dumfries DG1 3NE, Scotland

[9] Scotlands Rural Coll SRUC, Ctr Safe & Improved Food, Kings Bldg,West Mains Rd, Edinburgh EH9 3JG, Scotland

来源：

BIORESOURCE TECHNOLOGY | 2023年 / 386卷

关键词：

Lignocellulosic biomass; Pretreatment process; Nanocatalyst; Cellulolytic enzymes; Reducing sugars; ENHANCED HYDROLYSIS; CELLULASE; NANOPARTICLES; IMMOBILIZATION; PERFORMANCE; BIOMASS;

D O I：

10.1016/j.biortech.2023.129491

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The present investigation is targeted towards the facile fabrication of a carbon-based nanocatalyst (CNCs) using Kans grass biomass (KGB) and its sustainable application in microbial cellulase enhancement for the alleviation of enzymatic hydrolysis for sugar production. Different pretreatments, including physical, KGB extract-mediated treatment, followed by KOH pretreatment, have been applied to produce CNCs using KGB. The presence of CNCs influences the pretreatment of KGB substrate, fungal cellulase production, stability, and sugar recovery in the enzymatic hydrolysis of KGB. Using 1.0% CNCs pretreated KGB-based solid-state fermentation, 33 U/gds FPA and 126 U/gds BGL were obtained at 72 h, followed by 107 U/gds EG at 48 h in the presence of 0.5% CNCs. Further, 42 degrees C has been identified as the optimum temperature for cellulase production, while the enzyme showed thermal stability at 50 degrees C up to 20 h and produced 38.4 g/L sugar in 24 h through enzymatic hydrolysis of KGB.

引用

页数：9