One-pot green synthesis and characterization of copper oxide nanoparticles with antibacterial and antioxidant properties using Coleus amboinicus

The current research aims to employ an eco-friendly, less toxic, inexpensive method for the synthesis of copper oxide (CuO) nanoparticles with antibacterial and antioxidant potential using leaf extract of Coleus amboinicus which behaves as a reducing and capping agent. The size, functional group, shape, stability and purity of the as-synthesized CuO nanoparticles were analysed by FT-IR, XRD, TEM, EDX, TGA and zeta potential techniques. All characterization results displayed that the CuO nanoparticles are crystalline, spherical with an average size of 40-60 nm and stable. TGA curve denotes the weight loss of 16.6% that occurred in CuO nanoparticles due to the evaporation of moisture and volatile compounds. Moreover, the antioxidant and antibacterial properties of Coleus amboinicus-mediated CuO nanoparticles were assessed by DPPH assay and agar well diffusion method. Both assays confirmed and revealed that the synthesized CuO nanoparticles have good antibacterial activity against Escherichia coli and Salmonella sp. and antioxidant properties. It can be effectively used as a good antibacterial and antioxidant agent in various medical applications.