Cryobacterium zhongshanensis sp. nov., an actinobacterium isolated from Antarctic soil

A Gram-staining-positive, heterotrophic, non-spore-forming, non-motile, rod-shaped, strain ZS14-85(T) belonging to the genus Cryobacterium was isolated from soil in Antarctica. Growth was observed in the presence of 0-2% (w/v) NaCl, at pH 7.0-9.0 (optimum, pH 7.0) and 4-30 celcius (optimum, 20 celcius). Phylogenetic analysis showed that strain ZS14-85(T) formed a lineage in the genus Cryobacterium. The digital DNA-DNA hybridization (dDDH) values between strain ZS14-85(T) and its close relatives Cryobacterium psychrotolerans CGMCC 1.5382(T), Cryobacterium soli MCCC 1K03549(T) and Cryobacterium breve NBRC 113800(T) were 22.5, 22.3 and 22.2%, respectively. Orthologous Average Nucleotide Identity (OrthoANI) scores between strain ZS14-85(T) and C. psychrotolerans CGMCC 1.5382(T), C. breve NBRC 113800(T) and C. soli MCCC 1K03549(T) were 78.7, 78.1 and 77.7%, respectively. The polar lipids of strain ZS14-85(T) were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unidentified glycolipid (GL) and two unidentified lipids (L). The major fatty acids were anteiso-C-15:0 (60.7%), iso-C-16:0 (17.0%) and anteiso-C-17:0 (15.2%). MK-10, MK-11 and MK-9 were the predominant respiratory menaquinones. Based on phenotypic, phylogenetic and genotypic data, a novel species, Cryobacterium zhongshanensis sp. nov. is proposed. The type strain is ZS14-85(T) (= CCTCC AB 2019396(T) = KCTC 49384(T)).