Comparative analysis of full-field OCT and optical transmission tomography

被引:0
|
作者
Alhaddad, Samer [1 ]
Thouvenin, Olivier [1 ]
Boccara, Martine [2 ]
Boccara, Claude [1 ]
Mazlin, Viacheslav [1 ]
机构
[1] PSL Univ, Inst Langevin, CNRS, ESPCI Paris, 1 Rue Jussieu, F-75005 Paris, France
[2] Sorbonne Univ, Inst Systemat Evolut Biodiversite ISYEB, Museum Natl Hist Nat, EPHE,UA,CNRS, CP 50,57 Rue Cuvier, F-75005 Paris, France
关键词
TISSUES; EYE;
D O I
10.1364/BOE.494585Journal
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This work compares two tomographic imaging technologies, time-domain fullfield optical coherence tomography (FFOCT) working in reflection and optical transmission tomography (OTT), using a new optical setup that combines both. We show that, due to forward-scattering properties, the axial sectioning and contrast in OTT can be optimized by tuning illumination. The influence of sample scattering and thickness are discussed. We illustrate the comparison of the two methods in static (morphology) and dynamic (metabolic contrast) regimes using cell cultures, tissues and entire organisms emphasizing the advantages of both approaches. (c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
引用
收藏
页码:4845 / 4861
页数:17
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