The aim of this study was to investigate the effect of camel chymosin and different types of starter cultures on the chemical, textural, proteolytic and lipolytic characteristics of semi-hard white cheese prepared from camel milk during ripening. Two treatments of chesses consisting of thermophilic starter culture camel cheese (CC-TC) containing Streptococcus salivarius subsp. thermophilus and Lactobacillus delbrueckii subsp. bulgaricus and the mixture of thermophilic/mesophilic yogurt starter culture camel cheese (CC-YC) containing S. salivarius subsp. thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lacticaseibacillus casei subsp. casei and Levilactobacillus brevis were manufactured. Results show that camel cheese which was produced with YC as a starter culture had more yield in comparison with TC. The chemical changes were significantly higher in CC-YC (P < 0.05). The hydrolysis of alpha(s1)-casein, beta-casein and formation of their degradation products was relatively high in CC-YC. The total FFAs were significantly (P < 0.05) changed during 60 days of ripening and between CC-TC and CC-YC. The main substrate of lipolytic enzymes from both CC-TC and CC-YC was short-chain fatty acids containing butyric, caproic, capric, lauric, myristic, myristoleic, palmitic in camel cheeses which was decreased during ripening. Whereas CC-TC showed maximum hardness, adhesiveness, stringiness. However, CC-YC showed maximum resilience, cohesiveness, springiness, gumminess and chewiness. Therefore, the use of thermophilic/mesophilic yogurt culture is recommended for the production of good quality semi-hard white camel cheese, considering the chemical and textural characteristics of the cheese.