Employing non-canonical amino acids towards the immobilization of a hyperthermophilic enzyme to increase protein stability

被引：5

作者：

Switzer, Hannah J. ^{[1
]}

Howard, Christina A. ^{[1
]}

Halonski, John F. ^{[1
]}

Peairs, Emily M. ^{[1
]}

Smith, Nolan ^{[1
]}

Zamecnik, Maddy P. ^{[1
]}

Verma, Sanjana ^{[1
]}

Young, Douglas D. ^{[1
]}

机构：

[1] William & Mary, Dept Chem, Williamsburg, VA 23185 USA

来源：

RSC ADVANCES | 2023年 / 13卷 / 13期

关键词：

GENETIC-CODE; BIOCONJUGATION; SELECTIVITY; CHEMISTRY; ALKYNE;

D O I：

10.1039/d3ra00392b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A carboxylesterase derived from Sulfolobus solfataricus P1 was immobilized onto an epoxy-activated Sepharose resin via non-canonical amino acids. The immobilized enzyme exhibited heightened performance in organic solvents, recyclability, and stability at room temperature for over two years. The incorporation of a non-canonical amino acid afforded a high degree of control over the bioorthogonal immobilization reaction. These results indicate that the specificity conferred by genetic code expansion produces advantages in protein immobilization and broadens the utility of such proteins to non-biological settings.

引用

页码：8496 / 8501

页数：6

共 50 条

[1] Non-canonical amino acids and protein dynamics
Antony, Edwin
METHODS, 2024, 226 : 161 - 161
[2] Non-canonical amino acids in protein engineering
Link, AJ
Mock, ML
Tirrell, DA
CURRENT OPINION IN BIOTECHNOLOGY, 2003, 14 (06) : 603 - 609
[3] Protein Modification Employing Non-Canonical Amino Acids to Prepare SUMOylation Detecting Bioconjugates
Williard, Alexander C. C.
Switzer, Hannah J. J.
Howard, Christina A. A.
Yin, Rui
Russell, Brent L. L.
Sanyal, Ritwik
Yu, Shaun
Myers, Trinity M. M.
Flood, Brian M. M.
Kerscher, Oliver
Young, Douglas D. D.
PHARMACEUTICS, 2022, 14 (12)
[4] Protein modification through non-canonical amino acids
Tirrell, David A.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 231
[5] Non-canonical amino acids in protein polymer design
Connor, Rebecca E.
Tirrell, David A.
POLYMER REVIEWS, 2007, 47 (01) : 9 - 28
[6] Non-canonical amino acids in protein design, evolution and analysis
Tirrell, David
BIOPOLYMERS, 2007, 88 (04) : 519 - 519
[7] Exploiting Non-Canonical Amino Acids to Alter Enzyme Substrate Specificity
Windle, Claire
Nelson, Adam
Pearson, Arwen
Berry, Alan
FASEB JOURNAL, 2015, 29
[8] Incorporation of Non-Canonical Amino Acids
Leisle, Lilia
Valiyaveetil, Francis
Mehl, Ryan A.
Ahern, Christopher A.
NOVEL CHEMICAL TOOLS TO STUDY ION CHANNEL BIOLOGY, 2015, 869 : 119 - 151
[9] Non-canonical amino acids as tools for protein medicinal chemistry
Tirrell, David
Fang, Katherine
Lieblich, Seth
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 251
[10] Non-canonical amino acids in the interrogation of cellular protein synthesis
Tirrell, David A.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245

← 1 2 3 4 5 →