Synthesis of Silver Nanoparticles From Cymodocea rotundata Leaf Extract and Their Biological Activities

Aim: Silver nanoparticles (AgNPs) are considered to be a very significant and intriguing type within the category of metallic nanoparticles, particularly in the context of their involvement in biological applications. The objective of this research is to use the green synthesis method in order to synthesize AgNPs by using the leaf extract of C. rotundata. Furthermore, the study aims to evaluate the antioxidant and anti-inflammatory properties of these nanoparticles. Materials and methods: Fresh and healthy specimens of C. rotundata were gathered from Palk Bay, Tamil Nadu, India, and afterward subjected to a thorough washing process using tap water. The cleaned materials were air-dried and then fragmented into small bits and finely ground. The ethanolic extract of seagrass was then combined with a solution containing 1 millimolar (mM) silver nitrate (AgNo3). The decrease of silver ions in the solution was frequently measured using a UV-visible spectrophotometer. Synthesized AgNPs were investigated for antioxidants by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and anti-inflammatory activity was measured by protein-denaturation assay. Results: The use of C. rotundata leaf extract in the green synthesis of AgNPs, in the presence of 1 mM AgNO3, led to a noticeable alteration in the colour of the mixture, transitioning from a pale hue to a brown shade. This change in colour serves as evidence of the reduction of AgNo3 ions to silver ions, thereby facilitating the creation of AgNPs. The duration of the bio-reduction process of silver ions in the reaction mixture was observed to be two hours. The antioxidant and anti-inflammatory activity showed promising activity for AgNPs. Conclusion: This study concluded that C. rotundata had antioxidant capabilities, and AgNPs derived from C. rotundata have potential use in pharmaceuticals and medication administration.