Background: Malocclusion, the misalignment of teeth within or between the upper and lower arches, is a significant global oral health problem among children. Children affected by malocclusion, such as dento-facial disharmonies, may experience mental distress significant enough to warrant psychological and psychiatric consultation. The knowledge, attitudes, and practices of parents play a crucial role in the early prevention and treatment of malocclusion of their children as if the children are financially and decisively dependent on their parents. This study aims to assess the association between knowledge, attitudes, and practices of parents regarding malocclusion in children residing in Yangon, Myanmar. Methods: This Cross-sectional study was conducted in Yangon, Myanmar with a convenient sampling method. The research included a sample of 103 parents, encompassing both males and females aged between 20 to 50 years having children aged between 6 to 12 years. Results: By linear regression analysis, we found that educational level, knowledge and attitude significantly associate parents' practices regarding children's malocclusion. Three key factors: The higher the level of education (beta = 0.379, p < 0.01), the more they adopted good practice. Secondly, compliance with Knowledge: Parents who possessed greater knowledge about malocclusion (beta = 0.747, p < 0.01) were more likely to engage in practices related to their children's oral health. Lastly, Positive attitudes toward addressing malocclusion (beta = 0.541, p < 0.01) were associated with increased engagement in practices aimed at promoting children's oral health. Conclusion: This study underscores the significance of knowledge and attitude in driving parents' practices concerning children's malocclusion in Yangon, Myanmar. To enhance children's oral health, targeted educational efforts and awareness campaigns for parents are essential. Empowering parents with knowledge and fostering positive attitudes can encourage them to take proactive steps in preventing and treating malocclusion in their children. Strengthening these initiatives is vital to improving oral health outcomes among the younger population in the region.